国家林业局林业公益性行业科研专项项目(201204307)
为探讨白皮松天然群体遗传结构在地理分布上的差异,利用7对SSR引物对5个白皮松分布区域的遗传结构进行了分析。结果表明:7对SSR引物在5个区域内476个单株中共检测到14个多态性位点。各区域间观测等位基因数(Na)、有效等位基因数(Ne)、Shannon’s 信息指数(I)、观测杂合度(Ho)、期望杂合度(He)、Nei’s期望杂合度(Nei’s)分别介于1.7143~2.1429、1.1942~1.571、0.1948~0.4954、0.1726~0.3116、0.1178~0.3325、0.1172~0.3307之间。白皮松遗传多样性水平总体较低,区域间差异较大;遗传多样性水平最高的区域为秦岭西侧群体,其次为大巴山区群体;太行山与吕梁山群体多样性水平相对较低。区域间的遗传分化系数Fst介于0.0138~0.2242之间,基因流Nm介于0.865~17.8646之间。遗传分化较大、基因流水平较低的区域均发生在秦岭西侧及其与其他区域之间。各区域间遗传相似系数在0.8416~0.9964之间,遗传相似度最高的群体为太行山与吕梁山区域,遗传距离最大的为太行山与秦岭西侧区域。白皮松多样性分布的中心主要存在于秦岭西侧和大巴山区域,因此应对该区域进行重点保护。
In order to explore the genetic structure differences of natural populations of Pinus bungeana in geographical distributions. Genetic structure of 5 distribution regions was studied using 7 pairs of SSR primes. The result showed that: 14 polymorphic loci were detected in 476 individuals among five regions. The observed number of alleles(Na), effective number of alleles (Ne), Shannon's information index (I), observed heterozygosity (Ho), expected heterozygosity (He) and Nei's expected heterozygosity (Nei's) were 1.7143~2.1429、1.1942~1.571、0.1948~0.4954、0.1726~0.3116、0.1178~0.3325、0.1172~0.3307 respectively. The genetic diversity was relatively low among regions but the difference was high. The regions with the highest genetic diversity were west side of the Qinling Mountain followed by the Daba Mountain; the Taihang and Lvliang Mountain were relatively low. Genetic differentiation coefficient (Fst) was between 0.0138 ~ 0.2242 and gene flow (Nm) was between 0.865 ~ 17.8646 among regions. The populations with high genetic differentiation and gene flow were west side of the Qinling Mountain and among the regions of west side of the Qinling Mountain and other regions. Genetic diversity center of Pinus Bungeana were mainly distributed in the west side of the Qinling and Daba Mountain, therefore, the protection of Pinus Bungeana shoud focus on these regions.
赵罕.白皮松天然群体遗传结构的地理变异分析[J].植物遗传资源学报,2013,14(3):395-401.
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