国家教育部博士点基金
Ph.D. Programs Foundation of the Ministry of Education of China
在小麦抗叶锈近等基因系TcLr45中扩增出一条凝集素类受体蛋白激酶基因片段。以该序列为靶序列进行3’和5’RCAE-PCR,获得该基因cDNA全长片段为2347bp,该序列具有完整的开放阅读框,共编码730个氨基酸,命名为LecRK-LR45。经软件分析, LecRK-LR45具有典型的凝集素类受体蛋白激酶结构,属于L类型。聚类分析结果表明,LecRK-LR45与山羊草、小麦(Lr34)中的凝集素类受体蛋白激酶基因具有较近的亲缘关系,与玉米、拟南芥的亲缘关系较远。该基因的克隆为研究小麦中凝集素类受体蛋白激酶的功能奠定基础。
A fragment of lectin-like receptor kinases gene was amplified in wheat leaf rust near-isogenic line TcLr45. The full length cDNA of the gene 2347bp was obtained using 3’ and 5’ RACE-PCR, based on the target sequence. This sequence had a full open reading frame, encoding 730 amino acids, named as LecRK-LR45. Software analysis showed that LecRK-LR45 had a typical structure of lectin-like receptor kinases, belonged to L type. Cluster analysis result indicated that LecRK-LR45 had a closer genetic relationship with lectin-like receptor kinases gene of Aegilops tauschii and wheat(Lr34), and farther with maize and arabidopsis. The cloning of this gene lies a foundation for researching the function of lectin-like receptor kinases in wheat.
刘春燕,张娜,郭楠,等. TcLr45凝集素类受体蛋白激酶基因克隆与序列分析[J].植物遗传资源学报,2013,14(3):486-491.
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