Powdery mildew and barley yellow dwarf virus (BYDV) are important diseases, which affect wheat production worldwide. Resent years, both diseases are simultaneously found in some areas of China. To solve this problem, a wheat-Thinopyrum intermedium translocation YW642, containing BYDV resistance gene Bdv2, and a wheat line CB037, containing powdery mildew resistance gene Pm21, had been used to cross and backcross in this study. Through the disease resistance tests and molecular marker-assisted selection, 22 BC1 plants and 51 F2 plants which contain both Bdv2 and Pm21 genes were obtained. These wheat materials showed highly resistant to both powdery mildew and BYDV. Agronomic trait assays displayed that those resistant plants possessing both Pm21 and Bdv2 genes were better than susceptible plants and the parents plants following inoculation with the pathogens. Thus, these resistant plants developed in the study can be used as good germplasm resources for wheat breeding with resistance to powdery mildew and BYDV.