国家科技攻关计划
为了在林木育种和良种推广过程中对杂种松的真实性进行简单、快速鉴别,本研究以我国南方培育推广的2个杂种松及其3个亲本树种为研究材料,通过近红外光谱仪扫描各树种的针叶来采集数据,最后采用PLS-DA(偏最小回归-判别分析)方法建模对杂种松及其亲本进行鉴别。研究结果显示:本研究中PLS-DA方法对杂种松具有较高的判别能力,可以较好地将研究的2个杂种松及其3个亲本树种区别开来。因此,近红外光谱结合PLS-DA判别分析方法可以用来分析鉴别松树中的杂种和纯种,相对于同工酶和分子标记这些种质鉴别方法,近红外光谱技术具有简便、快捷等优点,值得在林木种质判别分析中进一步研究利用。
It is not easy to distinguish pine hybrids and their parents using their morphological characteristics. The traditional method (chemical analysis or molecular marker technology) of identification is complicated, time-consuming and costly. The truth of pine hybrids must be confirmed during the program for forest tree breeding and popularization of good pine varieties. Therefore, partial least square-discriminant analysis(PLS-DA) regression modeling was constructed using the data of near-infrared spectroscopy of the pine foliage for 2 pine hybrids and their 3 parent species in southern China. The results showed that PLS-DA has a high accuracy rate , allowing a secure identification of 2 hybrids and their 3 parent species. It is clear, from the results, that near-infrared technology would be used to identify hybrids and purebreds in pine and it possesses the advantage of being simple,inexpensive,and easy to operate.
栾启福,李彦杰,姜景民.利用近红外光谱对杂种松及其亲本的鉴别分析[J].植物遗传资源学报,2013,14(6):1083-1088.
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