利用SSR标记构建了27份中国无籽西瓜主栽品种的DNA指纹并进行了遗传多样性分析。24对多态性引物共扩增出66种基因型,基因型数2-5个不等,平均2.75个。平均多态性信息量(PIC)为0.37,变化范围为 0.19~0.66。有4个品种具有特征谱带。27个品种遗传相似系数变化范围为 0.7045~1.0,平均0.8683。组合24对引物,除无法区分‘郑抗无籽1号’与‘雪峰花皮无籽’,其余品种均能一一区分开。采用类平均法进行聚类分析,在相似系数0.83处,可将27个品种分为3大类。
Simple sequence repeat (SSR) markers were used to construct the DNA fingerprinting database of 27 Seedless Watermelon Major varieties in China and the genetic diversity was also analyzed. 24 polymorphic primer pairs could amplify 66 genotypes, and 2.75 genotypes were detected by each primer pair with the range from 2 to 5. The PIC values ranged from 0.19 to 0.66 with the mean of 0.37. 4 varieties had unique bands. The genetic similarity coefficients of the 27 varieties ranged from 0.7045 to 1.0 with the mean of 0.8683. Besides ‘Zhengkangwuzi No1’and‘Xuefenghuapiwuzi’, the other varieties could be separated clearly from each other. Furthermore, all the varieties were classified into 3 groups, at the level of genetic similarity coefficient 0.83 according to cluster analysis by an un-weighted pair-group average method with arithmetic mean.
赵胜杰,朱红菊,路绪强,等.无籽西瓜品种SSR指纹图谱构建及遗传多样性分析[J].植物遗传资源学报,2013,14(6):1142-1146.
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