A gene, named as TaUES (up-regulated expression under saline-stress in wheat, ID: KC408382) expressing significantly after NaCl treatment for 24hrs, was cloned from the leaves of wheat variety Shanrong No.3 based on the results of wheat gene chips, and it encoded a cysteine-rich polypeptide with 97 amino acids, containing a BowB domain and 10 conserved cysteine residues. The analysis of amino acid sequence indicated that this polypeptide showed high homologue to those of BBI-type protease inhibitors from wheat or other species, implying that TaUES may be a gene encoding a new wheat BBI-type protease inhibitor. TaUES expression suggested that it was involved in salt and drought stress response. Preliminary analysis of ectopic gene expression showed that over-expression of TaUES in transgenic tobacco lines resulted in their high tolerance to salt stress.