Genetic diversity of 194 sweet sorghum germplasm resources with different origins was analyzed based on agronomical traits using analysis for agronomical variance and cluster analysis. It is indicated that variation of agronomical traits was obvious among germplasm resources of different origins. Exotic germplasm resources could be used for germplasm enhancement because of their elite characters such as high plant height, high biomass and high sugar content compared to domestic ones. All the germplasm resources were clustered into 6 groups at the genetic distance of 0.66 mainly according to their agronomical traits. Information of this study can be used to select parent lines for development of segregating populations and hybrid breeding of sweet sorghum.