浙江省重大科技项目,2012C12903, 浙江省农科院创新提升工程项目2012R23Y01E01
本实验以从100份茄子种质资源中低温筛选出的耐低温基因型材料E659和耐低温敏感基因型材料E646为试材,研究了低温处理对不同耐寒性茄子叶片光抑制能力,丙二醛含量和电导率,过氧化氢酶(CAT)、抗坏血酸酶(APX)和超氧化物歧化酶(SOD)活性的影响。结果表明,在4℃处理5天后,与低温敏感材料E646相比,低温胁迫下筛选获得的极耐低温基因型E659, FV/FM 比值下降较少,表明其PSII 系统受损较少,而丙二醛含量和电导率上升幅度较低则表明膜脂过氧化反应引起的氧化损伤较轻。4℃低温处理24小时之内,发现E659植株中抗坏血酸酶活性增高,过氧化氢酶和超氧化物歧化酶的活性损害减弱或时间推迟。可见,茄子耐低温基因型E659在低温胁迫下能快速启动应激反应,增强氧化系统清除能力,来减轻低温对植株生长发育的伤害。
We obtained 1 a low temperature tolerant cultivar E659 by screening from 100 eggplant germplasm resources under low temperature stress. E659 and E646, a cold sensitive cultivar were subsequently Two eggplant genotypes, E659 and cold sensitive cultivar E646, were analyzed for low temperature tolerance to low temperature during early vegetative growth. Through measurements of photochemical capacity, oxidative damage, hydrogen peroxide accumulation, and antioxidant enzyme activities. After 5days of 4°treatment , compared with E646, the low temperature resistant genotype E659 showed, less decrease of FV/FM ratio and less increase of Malondialdehyde(MDA) content and electrolyte leakage, respectively, demonstrating minor damage of PSII system and oxidative damage by lipid peroxidation in this cultivar. Furthermore, within 24 hours of 4℃ treatment, the measurement of the antioxidant enzymes activity showed E659 has higher constitutive superoxide dismutase (SOD), and reduced or delayed damages of the ascorbate peroxidase (APX) and catalase (CAT) activity. These finding indicate that the low temperature tolerant cultivar E659 can possesses a rapid response mechanism and has higher reactive oxygen species system(ROS) clearance capacity to lower cold injury when facing low temperature.
包崇来,杜黎明,胡天华,等.茄子耐低温材料的筛选及其耐低温生理响应研究[J].植物遗传资源学报,2013,14(6):1161-1166.
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