作物种质资源保护项目(NB2013-2130135-19);青海省普通高等学校研究生创新研究项目(青学委〔2012〕)
为了研究“阿勃”在青海省不同生态区种植的广适性,本实验采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,以青海省不同生态区15份 “阿勃”为材料进行高分子量麦谷蛋白亚基(HMW-GS)的遗传多样性分析。结果表明:“阿勃”HMW-GS的亚基组合类型有8种,主要为1/7+8/2+12;有11种亚基,各个亚基出现的频率范围6.67%-100%;Glu-B1位点遗传多样性指数较大,为0.239;供试材料群体间GS的变化范围为0.35-1,平均为0.675,所有“阿勃”聚为三大类,其中13份“阿勃”材料聚在第Ⅰ大类,另两份分别聚在第Ⅱ和第Ⅲ大类,说明在青海不同生态区本土种植保留下的“阿勃”HMW-GS遗传多样性丰富。
In order to study the widely adaptation and detect the genetic diversity of Abodanza(Triticum.aestivum L.) in the different ecological area of Qinghai ,subunits composition of high mplecular weight gluten with 15 Abodanza were analyzed by means of SDS- PAGE. The results were described as following: A total of 8 allele combinations were observed in the 15 Abodanza and the predominant type was 1,7+8 and 2+12;there were 11 alleles on Glu-1 locus with the frequencies varied from 6.67% to 100%;The values of D of the Glu-B1 loci were higher and Glu-B1 loci has abundant genetic diversity. The result showed that genetics similarities ranged from 0.35 to 1 with an average of 0.675. All materials were divided into 3 groups and 13 wheat materials Abodanza were clustered into the first group while another two materials were respectively clustered into the second group and the third group .These results indicated that Abodanza materials have abundant genetic diversity.
王晓辉,李风珍,李高原,等.青海省小麦品种阿勃高分子量麦谷蛋白遗传多样性研究[J].植物遗传资源学报,2014,15(1):84-88.
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