153 bands were generated among 75 radish germplasms using 22 pairs of SSR primers which were screened out. Of them, 87 bands were polymorphic. The percentage of polymorphic alleles was 55.49%. On average, the number of alleles per SSR locus was 6.95 in total and 3.95 of polymorphic bands The genetic diversity among these radish germplasms was rich. After raw data processing and filtering by MATLAB, 75 radish germplasm could been distinguished and their molecular identities were constructed usingr 8 pairs of SSR primers. It showed that it is feasible to employ SSR markers to construct the molecular identities for elite germplasm resource and new varieties in radish.