利用SSR分子标记技术构建3个特早熟甘蓝型油菜恢复系的指纹图谱,筛选出这3个特早熟甘蓝型油菜恢复系的共显性SSR标记,测定了这3个恢复系的异交率。结果表明,恢复系材料4395、3509、4152的异交率分别为46.02%、33.32%、18.12%,在p=0.01时呈极显著差异,说明3个特早熟甘蓝型油菜恢复系的异交差异明显,为确定3个特早熟甘蓝型油菜恢复系在综合杂交种中比例高低提供依据。
Abstract:Fingerprinting of three very early Brassica napus restorer lines by using SSR molecular markers . CO dominant SSR markers were found in the 3 early maturing B.napus restorer lines and their F1; Those primers were used to determine the outcrossing rate of these 3 restorer lines in fields. The results showed that the outcrossing rate of these restorer lines (4395, 3509, 4152 ) were respectively in 46.02%, 33.32%, 18.12%, there was a significant difference in p = 0.01, it illustrated that the outcrossing rate of three very early maturing B.napus restorer lines had differences; And It provided a basis that to determine the proportion of three very early Brassica napus restorer lines in Synthetic hybrids.
李开祥,杜德志.特早熟甘蓝型油菜异交率的测定[J].植物遗传资源学报,2014,15(5):1156-1160.
复制