Interspecific hybridization is an important approach to broaden genetic base and obtain new species and germplasm. The objectives of this study were to obtain the interspecific hybrids from B. juncea and B. capitata and to study their morphological and cytological characteristic. The results showed that distinct differences between remained ovaries and obtained embryos existed after 30d in different crosses which made the influence on the obtaining of embryos. 36 true hybrids were identified by morphology method, which showed strong vegetative heterocyst. In addition, the meiosis behavior of the true hybrids was unnormal and some chromosomes lost in different degrees in meiosis anaphase. Some clones had 27 chromosomes and others were from 27 to 34. The pollen fertility of the true hybrids ranged from 0 to 47.87% and the self-fruitful rate was between 0 and 3.65%.