In order to know the performance of different genotypes of soybean germplasms at flowering-podding stage, explore drought-resistant resource, we determined 18 morphological and biochemical indexes including stem thickness, branch No.and plant height in the pot experiment at flowering-podding stage with 22 soybean varieties(lines) widely grown under artificial water stress condition. Drought resistances of varieties were scored with drought coefficient method and principle component analysis and cluster under water stress. The result showed that there were certain variations of drought coefficient of 18 morphological and biochemical indexes under water stress and variation coefficient was more than 10%. The accumulative contribution rate of 5 comprehensive principle components was 84.82%, which could stand for 84.82% of primitive information of soybean drought resistance. Using drought-resistant balancing D-value, 22 different eco-type soybeans were divided into 3 types: high tolerant, moderate tolerant and weaker tolerant. In 22 varieties (lines), we screened out the high drought tolerant varieties consisting of ‘Jinda78’ , ‘Jinda74’ ,’Jinda70’ ,and ‘Jinda73’.The varieties (lines) ‘Jinda75’, ‘Bianjing’ and ‘Heizhenzhu’ had weaker drought resistance. The rest had medium drought resistance. We could avoid one-sidedness and instability by using principle component analysis and fuzzy cluster than single trait. The comprehensive D-value was a method for soybean drought resistance comprehensive evaluation and drought-resistant cultivars breeding, it could be used to identify soybean drought resistance.