国家重点基础研究发展计划(973计划)
The National Basic Research Program of China (973 Program)
摘要:鉴定和克隆水稻穗顶部退化突变体新基因,对研究小穗顶部退化的分子机制及克服育种和生产实践中因穗顶部退化引起的产量损失具有重要的理论和现实意义。本研究报道了一个来源于中花11的组织培养的穗顶部退化突变体,暂命名为Panicle Apical Abortion 2(paa2)。该突变体的穗顶部小穗发育异常、退化,后期退化部分脱落,稻穗形成秃尖,使穗粒数减少。遗传分析表明该突变体受一个显性基因控制。利用群体分离分析法(bulked segregation analysis, BSA)将PAA2基因定位在2号染色体的长臂端L2-33和L2-50之间,物理距离为80 kb的范围内。该研究结果为PAA2基因的图位克隆奠定了基础。
Abstract::The identification and cloning of novel gene controlling rice Panicle Apical Abortion (PAA) is meaningful to understand underlying the molecular mechanism of the trait in order to reduce grain yield losses caused in rice production. We here reported the result of fine mapping to a rice mutant, paa2, derived from tissue culture of Zhonghua 11. Phenotypic analysis found that mutant panicle apical is gradually degraded during the panicle development. Genetic analysis revealed that the paa2 is caused by single dominance gene mutation. Using the BSA method, the PAA2 gene was finally mapped within the interval of 80 kb on chromosome 2, flanked by the newly developed markers of L2-33 and L2-50. The results provided here would beneficial the map-based cloning of PAA2 gene.
李真,毛毕刚,衡月芹,等.水稻穗顶部退化基因PAA2的精细定位[J].植物遗传资源学报,2014,15(5):1023-1027.
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