在对小麦全长cDNA克隆进行大规模测序及转录因子功能研究过程中,筛选到一个盐胁迫相关的bHLH转录因子基因,将其命名为TabHLH13。TabHLH13的全长cDNA序列为1072bp,开放阅读框为720 bp,编码一个具有240个氨基酸残基的bHLH转录因子;对TabHLH13的基因组和cDNA序列比较分析表明该基因包括5个外显子和4个内含子;同源序列分析发现,TabHLH13与来自大麦和短柄草中的bHLH蛋白序列相似性最高,分别为96.2%和90.5%;电子定位发现TabHLH13在小麦第七同源群的7DL上;亚细胞定位结果表明,TabHLH13编码一个定位在细胞核中的蛋白;组织表达特性分析表明该基因在小麦根、茎、叶、雌蕊和花药中均有较强的表达;半定量RT-PCR与qRT-PCR结果表明TabHLH13是一个受盐胁迫诱导表达的基因。
In the large-scale sequencing of the wheat full length cDNAs clones in our laboratory and functional analysis of transcriptional factors, a salt stress related gene was screened out and named TabHLH13. TabHLH13 is 1072 bp in full length with a 732 bp ORF, encoding a bHLH transcriptional factor with 240 amino acids. After comparing the genomic sequence with its cDNA sequence of TabHLH13, we found that it contains five exons and four introns. Homologous analysis found that TabHLH13 had a similarity with bHLH proteins from barley and Brachypodium as high as 96.2% and 90.5%, respectively. Using electronic mapping strategy, TabHLH13 was mapped onto wheat chromosome 7DL. The results of subcellular localization indicated that the TabHLH13 protein was a nuclear-localized protein. Tissue specific analysis indicated that TabHLH13 expressed in root, stem, leaf, pistil and anther. RT-PCR and qRT-PCR revealed that the expression of TabHLH13 was induced by salt stress.
符思路,刘国祥,张立超,等.小麦盐胁迫相关基因TabHLH13的克隆与分析[J].植物遗传资源学报,2014,15(5):1006-1011.
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