国家高技术研究发展计划(863计划);国家自然科学基金项目(面上项目,重点项目,重大项目)
The National High Technology Research and Development Program of China (863 Program);The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
为挖掘玉米分子伴侣基因ZmBiP1的功能,本研究通过荧光定量PCR技术研究ZmBiP1基因的表达模式,并将ZmBiP1基因转入拟南芥中研究其基因功能。荧光定量PCR结果表明,ZmBiP1基因在玉米旱21的不同组织器官中均有表达,在子房中表达量最高。ZmBiP1基因受甘露醇胁迫诱导诱导上调表达,受盐胁迫诱导下调表达。转基因拟南芥功能验证结果表明,过表达ZmBiP1基因导致拟南芥在种子萌发时期对盐和甘露醇胁迫的耐受能力减弱。
Molecular chaperone ZmBiP1 gene were identified and characterized in maize "Han21" line. The quantitative RT-PCR analysis indicated that ZmBiP1 gene had different expression patterns in different organs. It was highly expressed in ovaries. ZmBiP1 showed an up-regulated expression under mannitol conditions and down-regulated expression under NaCl conditions. Overexpression of ZmBiP1 in Arabidopsis led to sensitivities to salt and mannitol stresses in seed germination periods. The results suggested that ZmBiP1 gene may be a negative regulator in stress signaling pathways.
宋仲戬,张登峰,李永祥,等.玉米分子伴侣基因ZmBiP1的表达模式和功能研究[J].植物遗传资源学报,2015,16(3):576-580.
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