利用SCoT分子标记技术对来自9个国家或地区的47份菠萝种质进行了遗传多样性分析,并对SCoT标记在菠萝研究中的效率做了探讨。结果表明,SCoT标记在菠萝种质中具有丰富的多态性,引物多态性条带百分比在30%~100%之间,平均为85.37%;引物的有效等位基因数(Ne)、Nei’s基因多样性指数(H)、Shannon’s信息指数(I)和多态性信息含量(PIC)平均值分别为1.45、0.27、0.41和0.28,表明SCoT标记具有较高的多态性检测效率。基于SCoT标记计算获得的遗传相似系数对菠萝种质做了聚类分析,47份菠萝种质可被划分为6个类群,其中,第Ⅰ、Ⅱ、Ⅲ类群所包含的菠萝种质数量最多,占菠萝种质总数的80.85%。主成分分析获得了与聚类分析不尽一致的结论,但两者反映的种质亲缘关系基本一致。本研究结果将为我国菠萝种质的鉴定、保存和科学利用提供一定的理论依据。
The genetic diversity of forty seven originated from nine countries/regions were analyzed using SCoT molecular marker and the efficiency of SCoT marker in pineapple study was also discussed. Results showed that SCoT has the plenty polymorphsim in pineapple, the percentage of polymorphism bands ranged from 30%-100% and the mean value was 85.37%. The mean value for effective number of alleles, Nei’s gene diversity, Shannon’s information index and polymorphism information content was 1.45, 0.27, 0.41and 0.28, respectively, which suggested that SCoT has the high efficiency on detecting polymorphism in pineapple. Based on the genetic similarity coefficient between pineapple germplasm generated from SCoT markers, all the germplasms were divided into six groups. The first three larger group were groupⅠ, groupⅡand group Ⅲ, which account for 80.85% of the total pineapple germplasm. Although results obtained by principal component analysis was incomplete with that obtained by cluster analysis, the genetic relationship between pineapple germplasm illustrated with the two methods was roughly consistent. All these results would provide the important theoretical basis for the identification, protection and scientific utilization of pineapple in China.
王健胜,贺军虎,陈华蕊,等.菠萝种质目标起始密码子(SCoT)遗传多样性分析[J].植物遗传资源学报,2015,16(4):848-856.
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