农业部物种资源保护(农作物)项目
species resources protection (crops) project of MOA
摘要:本研究利用98对SSR标记对202份中国栽培稻地方种和选育种的遗传多样性进行比较分析。结果显示供试品种具有较丰富的遗传多样性,共检测到等位基因1350个,每个位点的等位基因数(Na)变化范围为3-39,平均14个;Nei基因多样性指数变化范围(He)为0.125-0.955,平均0.733;多态信息量(PIC)变化范围为0.122-0.953,平均0.680;稀有等位基因数(Nr)913个;等位基因丰度(Rs)8.33。栽培稻地方种和选育种遗传多样性差异明显,地方种等位基因数、Nei基因多样性指数、多态信息量、稀有等位基因数和等位基因丰度(Na=1219,He=0.747,PIC=0.710,Nr=756,Rs=8.50)均高于选育种(Na=919,He=0.704,PIC=0.650,Nr=529,Rs=7.01)。各染色体组水平的遗传多样性分析表明,选育种仅在1号染色体上的遗传多样性高于地方种,进一步分析显示选育种的遗传改良在基因组水平上具有区间特异性。
Abstract: Ninety-eight SSR markers were used to compare the genetic diversity in 202 Chinese cultivated rice varieties. The result showed a high level genetic diversity in Chinese cultivated rice. All the 98 loci revealed a total of 1350 alleles. The number of allele per locus (Na) ranged from 3 to 39, with an average of 14. The Nei’s genetic diversity index of per locus (He) was 0.733 with a range from 0.125 to 0.955. The polymorphism information content (PIC) varied from 0.122 to 0.953, with a mean of 0.680. Number of rare alleles (the allele frequency < 5%) (Nr) was 913 and genetic richness (Rs) was 8.33. There exited a significant difference in genetic diversity between landrace and modern rice varieties. Landrace (Na=1219, He=0.747, PIC=0.710, Nr=756, Rs=8.50) had more variation than modern rice (Na=919, He=0.704, PIC=0.650, Nr=529, Rs=7.01). Diversity analysis in chromosomes demonstrated that the Chromosome 1 of modern rice had higher genetic diversity than that of landrace. Further analysis showed interval specificity of genetic improvement in the level of whole genome for modern rice.
邓宏中,王彩红,徐群,等.中国水稻地方品种与选育品种遗传多样性的比较分析[J].植物遗传资源学报,2015,16(3):433-442.
复制