Distinctness, uniformity and stability (DUS) of plant variety is one of prerequisites for granting Plant Breeders’ Rights. DUS test guidelines is the technical basis for DUS testing. In this paper, the process of the development of test guidelines for Job’s tears, including the subject, quantity requirements of propagation materials, selection of characteristics, identification of states of expression, screening of example varieties, assessment criteria of DUS and the design of technical questionnaire were elaborated. The development of DUS test guidelines for Job’s tears will contribute to improve plant variety protection, promote variety innovation and strengthen variety management for Job’s tears.