山西省青年科技研究基金项目(2013021024-5);现代农业产业技术体系(CARS-09);山西省农业科学院院育种基础项目(Yyzjc1519)
利用663份山西省普通菜豆资源并基于14个农艺性状,采用比较不同分组原则、取样比例和总体取样量的不同组合的取样方法,确定了“地理来源+平方根比例+20%总体取样量”为山西初级核心种质构建的方法。同时,在此基础上对663份资源中的一些具有极端性状的资源进行选择,最终确定了包含152份普通菜豆作为山西普通菜豆初级核心种质。通过总体与初级核心种质资源多样性分析,数量性状均值比较、数量性状极值、变幅和标准差比较,性状多样性的差异性分析和各性状总体分布的χ2检验,最后得出:152份普通菜豆资源能够代表山西省普通菜豆资源的总体,可作为山西省普通菜豆育种、评价和创新利用的优先样品集。
Based on 14 agronomic traits, the information of 663 germplasm resources of common bean stored in Shanxi Gene Bank were used to construct the primary core collections. In this paper, the different combinations of grouping rules, sampling proportion and total sampling amount were compared. As a result, the combination of geographical origins, proportions of square root and 20 percent of total sampling amount was used. Meanwhile, the germplasm resources including extreme characters were selected and eventually 152 common beans were confirmed as the primary core collections of Shanxi germplasm resources of common bean. By means of the diversity analysis, comparison of means, extreme value, variable amplitude and standard deviation of quantitative traits, and chi-square test of total distribution between total germplasm resources and primary core collections, the result showed that 152 common beans can represent the total common beans of Shanxi Gene Bank and can be used as the preferential studying objects of breeding, evaluation, improvement and usage.
郝晓鹏,王燕,田翔,等.基于农艺性状的山西普通菜豆初级核心种质构建[J].植物遗传资源学报,2016,17(5):815-823.
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