云南省科技计划项目(科技惠民专项)(2014RA153);科技领军人才培养(2016HA005);云南省应用基础研究计划青年项目(2015FD060);云南省花卉育种重点实验室开放(FKL-201504)
以34种野生秋海棠(包括4个变种)为试材,水稻(Oryza sativaL. ssp. japonica)为外标,采用流式细胞法测定其基因组大小,比较不同种、组之间基因组大小的差异,并分析与染色体数的相关性。结果表明:34种秋海棠基因组1C值在0.292~2.554 pg之间,最大值约为最小值的9倍,平均基因组大小为0.863 pg,最小的为盾叶秋海棠(Begonia peltatifolia),最大的为水鸭脚秋海棠(B. fomosana)。中国原产的30种秋海棠平均基因组1C值(1C=0.925pg)较南美洲原产的4种(1C=0.398 pg)大,台湾原产的3种1C值均比大陆原产的27种大。中国原产秋海棠不同组间基因组的大小存在差异,同一组内基因组大小亦不相同,本研究所测材料以四室组的基因组最大,为1.285 pg,组内变化近3.2倍;秋海棠组和二室组次之,分别为0.895 pg和0.888 pg,组内变化近6.4、6.8倍;侧膜胎座组基因组最小,为0.721 pg,组内变化约1.2倍。相关性分析表明秋海棠基因组大小与染色体数无显著相关性。本结果可为秋海棠遗传多样性分析及基因组学研究提供一定的基础数据。
Genome sizesof a Begonia collection comprising34 species (including fourvarieties) were screenedby flow cytometry, usingOryza sativa L. ssp. Japonica as anexternal reference, the differences in genome size between different speices and sections were compared, correlation between genome size and chromosome number was analysed. The results showed that 1C values varied between 0.292pg and 2.554pg, a 9-fold difference was found among genotypes with genome size, the meangenome size of 34 Begonia species was 0.863 pg, the genome of Begonia peltatifolia was smallest, the genome of B. fomosana origined from Taiwan was largest. The mean genome size of 30 Begonia species in China origin(1C=0.925pg) was lager than 4 Begonia species’ in South American origin(1C=0.398 pg), genome sizes of 3 Begonia species inTaiwan origin were all larger than Chinese origin Begonia species’. There were significant differences in the genome size between different sections of Chinese Begonias, and in the same section, the genome sizes of Begonias were not same. The mean genome size of Section Sphenanthera was largest, 1.285 pg, varied about 3.2 times; while Section Begonia, Section Platycentrum, a bit larger, which were 0.895 pg and 0.888 pg respectively, varied almost 6.4, 6.8 times; the meangenome size of Section Coelocentrum was smallest, 0.721 pg, varied about 1.2 times. Genome sizes were not positively correlated with chromosome number. The resu
杜文文,王祥宁,段青,等.34种秋海棠基因组大小比较与分析[J].植物遗传资源学报,2018,19(2):370-378.
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