Fatty acyl reductase are widely involved in the process of lipid metabolism, affecting anther development and cuticular wax sunthesis of male gametophytes. In this study, LbMS2-2 gene encoding fatty acyl reductase was isolated using RACE(rapid-amplification of cDNA ends) method from Ningxia wolfberry ( Lycium barbarum L.). LbMS2-2 consisted of an open reading frame (ORF) with length 1800 bp, which encoded a protein of 599 amino acid residues, and its isoelectric point was 9.00. Bioinformation analysis indicated that LbMS2-2 protein was located in the chloroplast. The amino acid sequence of LbMS2-2 shared high homology with fatty acyl reductase from Capsicum annuum、Nicotiana tabacum and Solanum tuberosum. Real-time fluorescence quantitative PCR results revealed that LbMS2 expressed highly in tetrad stage、single nucleus pollen stage and double nucleus pollen stage of anther development. The results of subcellular localization and in situ hybridization further confirmed that LbMS2-2 gene mainly detected in the tapetum and microspores of anther, and its protein was predominantly localized in chloroplast. As the result, the fatty acyl reductase is an important gene in the organ development of Lycium barbarum L.