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首页 > 过刊浏览>2018年第19卷第6期 >1180-1186. DOI:10.13430/j.cnki.jpgr.20180324001
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枸杞Lb14-3-3b基因的表达分析及转烟草研究
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宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021,宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021,宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021,宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021,宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021,宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室，银川 750021

基金项目:

国家自然科学基金（31560418）；国家自然科学基金（31360361）；国家自然科学基金（31360025）


Expression of Lb14-3-3b Gene in Wolfberry andTransformation into Tobacco
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Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021,Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021,Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021,Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021,Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021,Western Biological Resources Protection and Utilization Lab of National Education Ministry, College of Life Science, Ningxia University, Yinchuan 750021

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    摘要:

    在宁夏枸杞(Lycium barbarum L.)品种“宁杞1号”及其雄性不育突变体“宁杞5号”花药发育差异蛋白质组学研究的基础上,克隆了一个花药发育相关基因Lb14-3-3b,证实该基因在花器官中优势表达。本实验进一步利用荧光定量PCR技术分析枸杞Lb14-3-3b基因在花药发育过程中不同时期的表达特征,并通过构建植物过表达载体Lb14-3-3b-pCambia1305.1-35s,经根瘤农杆菌介导法转化模式植物烟草,探究该基因在植物生长发育中的功能。结果表明,在枸杞花药发育的各个时期,Lb14-3-3b都有表达,在花药二核花粉粒时期表达量最高。与野生型烟草相比,转Lb14-3-3b基因烟草生长发育迟缓,植株矮小,花器官畸变,花瓣缺少致使花型趋于四角化,雄蕊及花丝数目缺少且发育异常。推测枸杞Lb14-3-3b基因在烟草生长发育及花器官发育中起调控作用,研究结果为进一步探讨该基因在枸杞生长发育中的调控作用提供参考依据。

    Abstract:

    On the basis of the result of differential proteome study during the anthers development in wolfberry variety ‘Ning Qi No.1’ and its male sterile mutant ‘Ning Qi No.5’, we have isolated an anther development related gene Lb14-3-3b, and confirmed the gene is dominant expressed in floral organs. In order to further understand its expression characteristics in floral organs and functions related development of floral organs, we use real-time quantitative PCR approach to analysis Lb14-3-3b gene expression level at different stages of wolfberry anther development. At the same time, we constructed plant over-expression vector Lb14-3-3b-pCambia1305.1-35s and transformed it into model plant tobacco by method of Agrobacterium tumefaciens mediated. The results showed that Lb14-3-3b was expressed during all stages of the wolfberry anther development and the expression level highest at the stage of two-nuclear pollen grain. Compared with wild type, transgenic tobacco plants grew relatively slow and dwarf, floral organ distortion, such as one petals and stamen absent, four angle flower patterns. It is deduced that Lb14-3-3b gene might play a regulatory role in the process of tobacco plant growth and its floral organ development. It still needs to study on the growth and development of wolfberry to understand the regulated mechanism of 14-3-3 proteins. The results provide a reference for the further explore the regulation of the gene in the growth and development of Lycium barbarum L.

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徐蕊,周丽,李瑞国,等.枸杞Lb14-3-3b基因的表达分析及转烟草研究[J].植物遗传资源学报,2018,19(6):1180-1186.

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  • 收稿日期:2018-03-24
  • 最后修改日期:2018-07-12
  • 录用日期:2018-05-03
  • 在线发布日期: 2018-11-14
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