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首页 > 过刊浏览>2018年第19卷第6期 >1187-1196. DOI:10.13430/j.cnki.jpgr.20180425001
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裂叶榆叶绿体基因组及CP-SSR位点分析
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作者单位:

1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;,1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;,1河北农业大学林学院, 保定 071000;3河北省林业科学研究院, 石家庄 050061,1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;,1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;,1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;,1河北农业大学林学院, 保定 071000; 2河北省林木种质资源与森林保护重点实验室, 保定 071000;

基金项目:

国家自然科学基金(31370664) ；河北农业大学科研发展基金资助


Chloroplast Genome and CP-SSR Site Analyses of Ulmus laciniata
Author:
Affiliation:

1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;3 Hebei Academy of Forestry,Shijiazhuang 050061,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;,1Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding 071000 ;2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding 071000;

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    摘要:

    榆树材质优良，具有良好的耐旱、耐寒和耐盐碱能力，从温带、暖温带到亚热带都有分布。本研究对榆科植物（裂叶榆）的叶绿体基因组进行De novo测序，裂叶榆叶绿体基因组序列全长为158,953 bp，为典型的四段式结构，其中LSC区长度为88,032 bp，SSC区长18,846 bp，两个IR区长26,037 bp，GC含量为35.57%。裂叶榆叶绿体基因组总共编码139个基因，包括85个蛋白编码基因、8个rRNA和46个tRNA基因。裂叶榆的叶绿体基因组存在755个SSR位点，SSR序列长度主要以6 ~ 8 bp的短序列为主，SSR共有49个重复单元，以A/T和AT/AT为主，占所有SSR位点的66.09%。选取42个物种叶绿体基因组的共有蛋白编码基因进行系统进化分析表明，裂叶榆与大麻科、桑科物种亲缘关系最近，榆科与大麻科、桑科均属于荨麻目，与传统分类学相吻合。本研究报道了裂叶榆的叶绿体基因组序列，对以后的榆树的光合作用研究、CP-SSR引物开发、进化研究及叶绿体转基因工程等研究具有重要意义。

    Abstract:

    Elm (Ulmus) has a long history of using as high-quality hard wood and could grow in temperate, warm temperate, and subtropical regions for its strong ability to resist adversity (drought, cold, and salt). This study performed De novo sequencing of the chloroplast genome of Ulmus laciniata. The lengths of the chloroplast genome of U. laciniata is 158,953 bp, containing four distinct regions: a pair of inverted repeat regions (IR) of 26,037 bp, large single-copy region (LSC) of 88,032 bp, and small single-copy region (SSC) of 18,846 bp. The chloroplast genomes had a GC content of 35.57% and consisted of a total of 139 genes. Among the genes, 85 were protein encoding genes, 8 were rRNA genes and 46 were tRNA genes. A total of 755 SSR loci were found that consisted by 49 repeating units in Ulmus laciniata chloroplast genomes, most of the perfect SSR sequences were based on short sequence lengths of 6 bp ~ 8 bp short sequence. The large proportion of SSR repeat units were A/T and AT/AT (499, 66.09%). The phylogenetic analysis of 42 species with common protein coding genes of chloroplast genomes was constructed. The results showed that U. laciniata was closely related to the plants of Cannabaceae and Moraceae, which belong to Urticales, and coincided with the traditional taxonomy. This study is report on Ulmus laciniata chloroplast genomes, which has significance for understanding photosynthesis, evolution, primer development and chloroplast transgenic engineering.

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杜久军,左力辉,刘易超,等.裂叶榆叶绿体基因组及CP-SSR位点分析[J].植物遗传资源学报,2018,19(6):1187-1196.

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  • 收稿日期:2018-04-25
  • 最后修改日期:2018-06-02
  • 录用日期:2018-06-15
  • 在线发布日期: 2018-11-14
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