广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640,广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640
国家水稻产业技术体系(CARS-01-32);广东省现代农业产业技术体系创新团队(2017LM1075);广东省科技项目(2015B020231003,2015A020210081,2016B090918116,2016A050502030,2017A020224014);广东省农业科学院学科团队建设项目(201613TD)
Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,Plant Protection Research Institue, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640
选育和应用抗病品种是防治水稻细菌性病害最经济、有效的方法,抗性资源的筛选及利用是选育抗病品种的前提。本研究利用苗期喷雾法对国内59个水稻品种组合、318份国外稻种资源以及13个抗白叶枯病近等基因系进行了细菌性条斑病抗性评价。测定的59个国内水稻品种组合对测试的细菌性条病病原均表现出感病;在鉴定的318个国外稻种资源中,抗细菌性条斑病的材料有82个,其中高抗和抗的稻种资源如POPONG、IR63372-8、WEDA HEENATI、BALAYAN、GHARIBE等17份;在测试的13个抗白叶枯病近等基因系中,仅有IRBB5表现出抗细菌性条斑病,对白叶枯病抗谱较广的IRBB21、CBB23对测试的病菌表现出高感。应用SPSS统计软件,对95份水稻资源进行了白叶枯病和细菌性条斑病的抗性相关性分析,82个抗细菌性条斑病的稻种资源和13个抗白叶枯病近等基因系对细菌性条斑病和白叶枯病的抗性相关性分析结果分别为r值0.103和p值0.358>0.05 、r值0.527和p值0.064>0.05 ,表明测试稻种对两病的抗性没表现出相关性。利用xa5基因功能标记,对79个抗细菌性条斑病资源进行了基因检测,结果显示有30个品种含有xa5基因,其余49个不含xa5基因。本研究筛选出的49个抗性稻种资源对抗细菌性条斑病基因的挖掘以及品种的创制具有重要的价值。
Application of resistant varieties is one of the most economical and effective measure to control rice bacterial leaf streak (BLS). Screening and utilization of resistant germplasm is the fundamental work for developing resistance varieties. 59 of domestic rice varieties or hybrid combinations,318 of foreign germplasms and 13 of near-isogenic lines (NILs) with resistance to bacterial blight (BB) were evaluated on their resistance of BLS. Among the tested rice entries, all of domestic varieties showed susceptible to BLS. 82 of rice germplasms were highly resistant or resistant to BLS, including Popong、IR63372-8、Weda Heenati、Balayan、Gharibe etc. Only BB near-isogenic line IRBB5 was resistant to BLS, while IRBB21 and IRBB23 with broad spectrum resistance to bacterial blight were highly susceptible to BLS. 95 of rice germplasms were selected to analyze the association between the resistances of BB and BLS. Association analysis of 82 rice germplassms and 13 of BB NILs both showed no correlation between two diseases, with the index r =0.103 (P value 0.358>0.05) and index r=0.527 (P value 0.064>0.05) respectively. 79 of rice germplasms with the resistance to BLS were detected with functional molecular markers of xa5 the result indicates that 30 of the tested germplasms habour the xa5 gene, while the other 49 of tested entries do not have the tested gene, which could be the important resoures for the genes mining and the rice breeding with the resistance against BLS.
冯爱卿,陈深,杨健源,等.水稻品种资源对细菌性条斑病菌的抗性评价[J].植物遗传资源学报,2018,19(6):1045-1054.
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