棉花NHL基因家族鉴定及黄萎病菌胁迫下的表达分析

doi:10.13430/j.cnki.jpgr.20180522001

首页 > 过刊浏览>2018年第19卷第6期 >1154-1169. DOI:10.13430/j.cnki.jpgr.20180522001

棉花NHL基因家族鉴定及黄萎病菌胁迫下的表达分析
DOI:
                        10.13430/j.cnki.jpgr.20180522001
                    
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作者单位:教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001,教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学，河北保定 071001
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基金项目:国家自然科学基金(No. 31171597);河北省百名优秀创新人才支持计划

Genome-Wide Identification of NHL Gene Family and Expression Analysis in Cotton under Verticillium dahliae Stress

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Affiliation:

North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000,North China Key Laboratory for Crop Germplasm Resources of Education Ministry/ Key Laboratory for Crop Germplasm Resources of Hebei / Hebei Agricultural University, Baoding 071000

Fund Project:

the National Natural Science Foundation of China (No. 31171597); Hundreds of Innovative Talents Project of Hebei Province

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NDR1/NIN1-like基因家族是从拟南芥基因组数据库中发掘出来的与拟南芥NDR1基因和烟草HIN1基因序列同源的基因,已有研究证明拟南芥NHL基因可在抗病中发挥作用。本研究从海岛棉和陆地棉中分别鉴定出了100个、118个NHL基因,并对其进行生物信息学分析。海岛棉和陆地棉NHL基因家族成员几乎遍布棉花各条染色体上,并可分为3个亚组,都含有3个与拟南芥保守基序相似的保守结构。利用接菌后抗病陆地棉的转录组数据,分析陆地棉NHL基因家族各成员表达变化,发现在黄萎病菌胁迫下,有57个陆地棉NHL基因在根和茎中的表达都发生了明显的变化,其表达模式可分为3种类型：接菌前高接菌后表达下降、接菌前较高接菌后表达先降后升、接菌前低接菌后表达升高。第一类基因的表达在接菌后一直受到抑制,推测其在棉花抗黄萎病中起负向作用；后两类基因受黄萎病菌诱导表达升高,推测其在棉花抗病中起正向作用。本研究结果为分析成员较多的NHL基因家族在棉花抗黄萎病中的作用提供了理论依据。

Abstract:

Previous studies showed that Arabidopsis NHL (NDR1/HIN1-like) genes played roles in the disease resistance. By bioinformatics analysis, we identified 100 and 118 NHL genes from Sea Island cotton and upland cotton, respectively. The NHL family members from Sea Island cotton and upland cotton were found to be distributed almostly on all chromosomes. These genes can be divided into three subgroups, and all contained three conserved domains as observed with the conserved motifs of Arabidopsis NHL gene family. The expression patterns of NHL family members in upland cotton were analyzed on the basis of the previously published transcriptional data of the resistant upland cotton after inoculated with V. dahliae. Fifty-seven NHL genes represented obvious expression changes in the root and stem, with three types of and their expression patterns: (1) highly expressed before inoculation and then decreased after inoculation; (2) highly expressed before inoculation, but firstly decreased and then increased after inoculation; (3) lowly expressed before inoculation and then increased after inoculation. The genes (type-1) were negatively regulated in the cotton resistance to Verticillium wilt, while the genes (type-2 and type-3) were positively regulated. Thus, the accumulative results provided a theoretical basis for further analyzing the role of NHL gene family in cotton Verticillium wilt resistance.

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刘丽霞,刘正文,王国宁,等.棉花NHL基因家族鉴定及黄萎病菌胁迫下的表达分析[J].植物遗传资源学报,2018,19(6):1154-1169.

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收稿日期:2018-05-22
最后修改日期:2018-09-06
录用日期:2018-07-26
在线发布日期: 2018-11-14
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