1山西农业大学农学院, 太谷 030801;2山西农业大学农业生物工程研究所, 太谷 030801;3农业部黄土高原作物基因资源与种质创制重点实验室, 太原 030001;4杂粮种质资源发掘与遗传改良山西省重点实验室, 太原 030001
山西省重点研发计划项目(201603D221003-6);国家自然科学基金项目(31601369,31371693,31471556);山西农业大学科技创新育种基金(20132-23)
ZHANG Bin
1College of Agricultural, Shanxi Agricultural University, Taigu 030801; 2Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801; 3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030001; 4 Shanxi Key Laboratory of Resources and Genetic Improvement of Minor Crops, Taiyuan 030001LI Hong-ying
1College of Agricultural, Shanxi Agricultural University, Taigu 030801; 2Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801; 3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030001;HAN Yuan-huai
1College of Agricultural, Shanxi Agricultural University, Taigu 030801; 2Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801; 3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030001; 4 Shanxi Key Laboratory of Resources and Genetic Improvement of Minor Crops, Taiyuan 0300011College of Agricultural, Shanxi Agricultural University, Taigu 030801;2Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801;3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030001;4 Shanxi Key Laboratory of Resources and Genetic Improvement of Minor Crops, Taiyuan 030001
This work is supported by the key discipline foundation of Shanxi province (201603D221003-6), National Natural Science Foundation of China (31601369, 31371693, 31471556), and the scientific and technological innovation breeding programs of Shanxi Agriculture University (20132-23).
糜子(Panicum miliaceum L.)具有抗旱、耐瘠、适应性广、生育期短等特点,是我国北方地区的杂粮作物。本试验选用糜子品种伊选大红糜进行EMS化学诱变而构建突变体库,对获得的2333个M2代材料进行了突变频率和全生育期表型多样性等特征的分析。结果显示,所构建的糜子突变体库材料变异类型非常丰富,共发现103个形态性状突变株系,突变频率为4.4%,包括叶色、叶型、株高、生育期、育性、穗型、种皮颜色等性状,该突变体库的构建将为糜子功能基因组学研究和育种提供丰富的突变体资源和新型种质。
Broomcorn millet (Panicum miliaceum L.) is one of the minor crops in China, which represented resistant and tolerant against drought, lower-fertilizer and abiotic stresses as well as short life-cycle. Within this study, by generating an EMS-triggered mutant population by using broomcorn millet variety Yi-Xuan-Da-Hong-Mi, we analyzed the mutation frequency and phenotypic variation of 2333 M2 plants. Out of that, a total of 103 plants with visible phenotype differing with the parental line were identified, yielding 4.4% of mutation rate. These mutants showed modifications on any of leaf color, leaf shape, plant height, mature period, fertility, spike shape, seed-coat color and so on. Thus, these mutants would become a genetic resource being useful in deciphering the gene function and breeding for new varieties in Broomcorn millet.
张彬,王喆,陈利青,等.糜子 EMS 突变体库构建和突变体筛选[J].植物遗传资源学报,2019,20(2):370-376.
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