内蒙古农业大学生命科学学院/内蒙古自治区植物逆境生理与分子生物学重点实验室/内蒙古自治区抗逆植物遗传资源利用与分子改良科技创新团队，呼和浩特 010018
国家自然科学基金项目（31560199）
- YANG Qi
YANG Qi
College of Life Sciences, Inner Mongolia Agricultural University/Key Laboratory of Plant Stress and Physiology and Molecular Biology, Inner Mongolia Autonomous Region / Science and Technology Innovation Team for Remediation of Plant Genetic Resources and Molecular Improvement, Inner Mongolia Autonomous Region, Hohhot 010018
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在本站中查找 - NIU Xiao-cui
NIU Xiao-cui
College of Life Sciences, Inner Mongolia Agricultural University/Key Laboratory of Plant Stress and Physiology and Molecular Biology, Inner Mongolia Autonomous Region / Science and Technology Innovation Team for Remediation of Plant Genetic Resources and Molecular Improvement, Inner Mongolia Autonomous Region, Hohhot 010018
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在本站中查找 - WANG Rui-gang
WANG Rui-gang
College of Life Sciences, Inner Mongolia Agricultural University/Key Laboratory of Plant Stress and Physiology and Molecular Biology, Inner Mongolia Autonomous Region / Science and Technology Innovation Team for Remediation of Plant Genetic Resources and Molecular Improvement, Inner Mongolia Autonomous Region, Hohhot 010018
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在本站中查找 - LI Guo-jing
LI Guo-jing
College of Life Sciences, Inner Mongolia Agricultural University/Key Laboratory of Plant Stress and Physiology and Molecular Biology, Inner Mongolia Autonomous Region / Science and Technology Innovation Team for Remediation of Plant Genetic Resources and Molecular Improvement, Inner Mongolia Autonomous Region, Hohhot 010018
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College of Life Sciences, Inner Mongolia Agricultural University/Key Laboratory of Plant Stress and Physiology and Molecular Biology, Inner Mongolia Autonomous Region / Science and Technology Innovation Team for Remediation of Plant Genetic Resources and Molecular Improvement, Inner Mongolia Autonomous Region, Hohhot 010018
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
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本研究克隆了中间锦鸡儿一个CiUPF0114基因，该基因编码框长876 bp，编码291个氨基酸的蛋白质，预测等电点9.83，分子量32.13 kD。在干旱、脱水和冷处理后，CiUPF0114基因转录水平的表达量均明显上调。通过全基因组筛选，获得了29个来自于8种豆科植物以及模式植物拟南芥和水稻中的UPF0114基因家族成员，并对其进行了系统进化分析、基因结构和保守基序分析。系统进化分析将UPF0114基因分成A和B两个亚家族，A亚家族又分成A1和A2两个组，中间锦鸡儿CiUPF0114属于B亚家族成员。该研究为进一步验证CiUPF0114基因的功能奠定了基础。
This study reported the isolation of CiUPF0114 gene from Caragana intermedia, which contained an 876 bp open reading frame, encoding for a protein with 291 amino acids. The predicted isoelectric point and molecular weight of CiUPF0114 were 9.83 and 32.13 kD, respectively. The expression level of CiUPF0114 gene was significantly up-regulated under drought, dehydration or cold treatments. Furthermore by genome-wide screening, a total of 29 UPF0114 gene family members were identified from eight legumes, Arabidopsis and rice. The UPF0114 genes from were divided into subfamily A and subfamily B according to phylogenetic analysis. The A subfamily was further divided into two groups, A1 and A2. CiUPF0114, a gene from Caragana intermedia, belongs to the B subfamily. These results might lay the foundation for further study on the functions of CiUPF0114.
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杨杞,牛肖翠,王瑞刚,等.中间锦鸡儿 CiUPF0114 克隆与表达及豆科 UPF0114 基因家族成员分析[J].植物遗传资源学报,2019,20(3):760-768.
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- 收稿日期:2018-08-29
- 最后修改日期:2018-12-03
- 录用日期:2018-12-04
- 在线发布日期: 2019-05-13
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