1江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心/江苏省优质水稻工程技术研究中心,江苏南京 210014;2扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州 225009
国家重点研发计划“长江中下游粳稻优质高产高效新品种选育”(2017YFD0100403),江苏省农业科技自主创新资金项目[CX(18)2022],江苏省现代农业重点研发项目(BE2017368)
CHEN Zhi-hui
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009WANG Fang-quan
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009XU Yang
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009WANG Jun
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009LI Wen-qi
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009FAN Fang-jun
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009ZHONG Wei-gong
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009YANG Jie
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014; 2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 2250091Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014;2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009
the National Key Research and Development Program (2017YFD0100403), the Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)2022), and the Jiangsu Province Key Research and Development Program (Modern Agriculture, BE2017368
携带Wx-mp基因的水稻材料直链淀粉含量约为10%,其米饭柔软,富有弹性,深受市场欢迎。为探明该基因在水稻品种资源中的分布情况以及加快该基因利用进程,我们开发了该基因的功能标记,对我国南方242份粳稻品种资源进行了检测,发现26份品种资源携带Wx-mp基因,其中25份来源于江苏品种和育种中间材料,1份来源于上海;为验证标记检测的准确性,对26份材料进行了重测序,测序结果与标记检测结果完全吻合;对26份材料的直链淀粉含量进行测定,直链淀粉平均含量为9.2%,变异幅度3.3%,直链淀粉含量最低的品种为南粳505,含量为7.3%,直链淀粉含量最高的品种是南粳46,含量为10.6%,以上结果表明,软米基因Wx-mp主要分布在江沪一带。
The rice varities harboring Wx-mp genotype generally results in 10% of amylose, which brings elite eating quality (e.g soft and elastic) favorited by consumers in Yangtze River Region, China. In order to identify Wx-mp carrying rice germplasm in future breeding, a functional marker associating to Wx-mp has been developed. By tests in 242 japonica rice materials collected from southern China, twenty-six germplasms were found carrying Wx-mp gene. Out of that, 25 varieties or breeding materials were collected from Jiangsu, the others from Shanghai. By validation using Sanger sequencing, this newly-developed marker proved 100% accuracy within 26 samples. Furthermore, average content of amylose was 9.2%, with a range of 3.3%. The rice cultivar Nanjing 505 with the lowest amylose content was 7.3, while cv. Nanjing 46 showed a highest amylose content of 10.6%. Thus, by deployment of the new functional marker, this study unlocked the distribution of Wx-mp carrying cultivars that were was mainly found in Jiangsu and shanghai Province.
陈智慧,王芳权,许扬,等.软米基因Wx-mp在部分粳稻品种资源中的分布[J].植物遗传资源学报,2019,20(4):975-981.
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