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SbSBP5基因参与高粱对干旱胁迫的响应
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作者单位:

1山西省农业科学院旱地农业研究中心/黄土高原东部旱作节水技术国家地方联合工程实验室/有机旱作山西省重点实验室，太原 030031;2农业部黄土高原作物基因资源和种质创制重点实验室，太原030031;3山西省农业科学院高粱研究所，晋中 030600

基金项目:

本研究由山西省农业科学院优势课题组自选项目(YYS1705)；有机旱作山西省重点实验室(201805D111015)；山西省重点研发计划重点项目(201703D211010)；山西省农业科学院特色农业技术攻关项目(YGG17016)


SbSBP5 is Involved in Response to Drought Stress in Sorghum
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Affiliation:

1Dryland Agriculture Research Center, Shanxi Academy of Agricultural Sciences/National local joint engineering laboratory of water-saving techniques for dry farming in the eastern Loess Plateau/ Organic dry farming of Shanxi Province key laboratory, Taiyuan 030031;2Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031;3Sorghum Institute of Shanxi Academy of Agricultural Sciences, Jinzhong 030600

Fund Project:

Foundation project：the Optional Project of Advantage Research Group of Shanxi Academy of Agricultural Sciences (YYS1705)；Organic dry farming of Shanxi Province key laboratory(201805D111015)；the Key Program of KeySResearch and Development Projects of Shanxi province(201703D211010)；Characteristic Agricultural Science and Technique Foundation of Shanxi Academy of Agricultural Sciences(YGG17016)

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    摘要:

    SQUAMOSA PROMOTER BINDING PROTEIN box (SBP-box)基因编码一类植物特有的转录因子，其功能涉及植物生长发育的诸多过程，包括植物叶片的形态建成、胚胎发生、营养生长到生殖生长的更替等，在作物遗传改良方面具有重要的实际应用价值。本文克隆了高粱SbSBP5基因，分析了其序列特征及其在不同逆境下的表达模式。结果表明SbSBP5编码800个氨基酸，含有一个典型SBP 结构域，其启动子区含有MBRE，ARE和MBS等逆境相关顺式元件；SbSBP5可受PEG和外源ABA诱导上调表达，表达峰值时分别是对照的12倍和11倍，SbSBP5启动子在转基因拟南芥中可驱动GUS报告基因表达，并且其活性可受干旱和ABA诱导增强，分别是对照的3倍和4倍。本文结果进一步证明SbSBP5参与了植株对干旱和外源ABA的响应，为深入研究SbSBP5的功能奠定了基础。

    Abstract:

    SQUAMOSA PROMOTER BINDING PROTEIN-box (SBP-box) genes, that belong to plant-specific transcription factors, are involved in many important aspects including leaf?development, embryogenesis, vegetative and?reproductive?phase transitions. This family has important practical application in crop genetic improvement. In this study, we isolated a sorghum SBP-box gene named SbSBP5, and conducted the sequence analysis and the transcriptional pattern under different biotic stresses. SbSBP5 coded for a polypeptide of amino acids that contained a typical SBP domain. By analyzing the promoter sequence, several stress-related cis-elements including MBRE, ARE and MBS were detected. Twelve and 11-fold up-regulation of SbSBP5 was detected by PEG-directed drought treatment or applying exogenous abscisic acid. In transgenic Arabidopsis that expressed SbSBP5 promoter plus a GUS reporter, three and four fold of GUS activity were detected under drought or exogenous abscisic acid treatments, respectively. Thus, these results provided preliminary outcome of SbSBP5 responding to drought and exogenous abscisic acid, which might provide a basis for further unlocking its molecular function.

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常建忠,董春林,张正,等. SbSBP5基因参与高粱对干旱胁迫的响应[J].植物遗传资源学报,2019,20(5):1301-1308.

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  • 收稿日期:2018-12-10
  • 最后修改日期:2018-12-25
  • 录用日期:2019-01-03
  • 在线发布日期: 2019-09-17
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