中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程
公益性行业(农业)科研专项(201203014);国家科技支撑计划项目(2013BAD01B02);转基因生物新品种培育重大专项(2016ZX08001-002)
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement
Agro-scientific Research in the Public Interest Program of China (20120314); National Sci-tech Support Plan (2013BAD01B02); The Major Science and Technology Project to Create New Crop Cultivars using Gene Transfer Technology (2011ZX08001-002)
稻瘟病抗性基因 Pib、Pita、Pi5、Pi25 和 Pi54 等对我国不同稻区的稻瘟病菌表现广谱抗性,在 水稻育种中具有较高的利用价值。本研究利用各基因的功能性分子标记,检测并分析了 Pib、Pita、Pi5、Pi25 和 Pi54 在我国水稻微核心种质中的分布情况。结果显示,124 份种质携带 1~4 个目标基因,其中黄丝桂占 携带基因 Pib、Pita、Pi5 和 Pi54;南雄早油占和乌嘴红谷分别携带 3 个基因 Pita、Pi25 和 Pi54 及 Pita、Pi5 和 Pi54;叶里藏花和辽粳 287 等 35 份分别携带 Pi5 和 Pi54、Pita 和 Pi54、Pib 和 Pi54、Pib 和 Pita、Pi25 和 Pi54、Pib 和 Pi5、Pita 和 Pi5、Pita 和 Pi25、Pi5 和 Pi25;抚宁紫皮粳子和隆化毛葫芦等 86 份分别携带 单个目标基因。为了解我国水稻微核心种质的抗稻瘟病基因型,以及有效利用优异种质改良水稻抗瘟病性 提供了信息。
Five blast resistance (R) genes, Pib, Pita, Pi5, Pi25 and Pi54, which showed relative broad-spectrum resistance to Chinese Magnaporthe oryzae isolates, were surveyed in the mini-core collection of Chinese rice germplasm by using their functional markers. As a result, 124 accessions of germplasm were found to harbor 1-4 target genes, respectively. Among them, Huangsiguizhan contained four R genes including Pib, Pita, Pi5 and Pi54;Nanxiongzaoyouzhan and Wuzuihonggu each harbored three R genes Pita/Pi25/Pi54 and Pita/Pi5/Pi54, respectively;35 accessions including Yelicanghua and Liaojing 287, etc. each harbored two R genes with genotypic constitutions of Pi5 and Pi54, Pita and Pi54, Pib and Pi54, Pib and Pita, Pi25 and Pi54, Pib and Pi5, Pita and Pi5, Pita and Pi25, Pi5 and Pi25, respectively. 86 accessions including Funingzipijingzi and Longhuamaohulou, etc. each harbored a single target gene. These results will provide references for grasping blast R genotypes of themini-core collection of rice germplasm and improving cultivar resistance using the elite accessions of germplasm.
岂长燕,许兴涛,马建,等.抗稻瘟病基因 Pib、Pita、Pi5、Pi25 和 Pi54 在我国水稻微核心种质中 的分布[J].植物遗传资源学报,2019,20(5):1240-1246.
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