甘肃省农业科学院作物研究所
国家自然科学基金项目(31760390,31860384);国家重点研发计划(2018YFD0100202-3,2016YFD0100103-19)
Crops Research Institute, Gansu Academy of Agricultural Sciences
The Natural Science Foundation of China (Grant No. 31760390,31860384),The National Key Research and Development Program of China (Grant No. 2018YFD0100202-3,2016YFD0100103)
叶色突变体是研究光合作用及叶绿体发育的重要材料。本研究以黄绿叶突变体ygl-F17138为材料,与玉米自交系B73进行杂交构建F2分离群体,进行遗传效应分析和基因初步定位。遗传分析表明,该突变性状由单个隐性核基因控制,且能稳定遗传。利用BSR-seq结合连锁分析的方法将该基因初步定位在第3条染色体上一个约9.2 Mb的区间内(chr3: 173087201-182203992),查询该区间内已知基因功能注释,未发现类似前人报道的调控黄绿叶性状基因,说明YGL-F17138基因是一个控制玉米黄绿叶发育候选基因。
Leaf-color mutants are important materials for deciphering the photosynthesis and chloroplast development. In this study, a yellow-green leaf mutant was identified and designated ygl-F17138. By making a cross with the maize inbred line B73, we constructed a F2 segregation population for the genetic analysis and the preliminary gene mapping. Genetic analysis indicated that this yellow-green phenotype was genetically controlled by a recessive nuclear gene. By deployment of BSR-seq and the linkage analysis method, this gene was allocated to an interval of about 9.2 Mb on chromosome 3 (chr3: 173087201-182203992). According to the functional annotation of known genes in this interval, no genes which regulating yellow green leaf traits similar to those previously reported were found, indicating that YGL-F17138 gene is a candidate gene controlling yellow green leaf in maize.
刘忠祥,寇思荣,连晓荣,等.玉米黄绿叶突变体表型鉴定及基因初步定位[J].植物遗传资源学报,2020,21(2):452-458.
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