By successive backcrossing and subsequent selection of the synthetic interspecific hybrids and allopolyploids with the cultivated species, the introgression of targeted chromosomal segments from the relatives becomes feasible. In this review, the intertribal hybridizations between the cultivated Brassica species and several distant relatives, cytogenetic behaviors and generation of new germplasm resource in our group are summarized. Due to the partial elimination of the chromosomes from pollen parents, the hybrids are able to maintain the whole or majority of the chromosomes from the female brassicas with the addition of few alien ones, and could produce the introgression lines with some male-derived characters, exhibiting the genetic and epigenetic alterations but with the same chromosome number as the female. The perspectives for crosses with distant relatives aiming at the new germplasm innovation are discussed.