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首页 > 过刊浏览>2020年第21卷第4期 >938-944. DOI:10.13430/j.cnki.jpgr.20191205001
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长江中游浮萍种质资源鉴定及遗传多样性分析
作者:
作者单位:

1.南京农业大学生命科学学院;2.中国热带农业科学院热带生物技术研究所/农业农村部热带作物生物学与遗传资源利用重点实验室

基金项目:

海南省重点研发计计划（ZDYF2018241），中国热带农业科学院基本科研业务费专项资金（ZDYF2018241，1630052016009）


Identification and Genetic Diversity Analysis of Duckweed Germplasm Resources in the Middle Reaches of the Yangtze River
Author:
Affiliation:

1.College of Life Science, Nanjing Agricultural University;2.Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources Utilization, Ministry of Agriculture and Rural

Fund Project:

Hainan Province Key R & D Plan（ZDYF2018241），Special Fund for Basic Scientific Research Business Expenses of Chinese Academy of Tropical Agricultural Sciences（ZDYF2018241，1630052016009）

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    摘要:

    浮萍为浮萍科水生植物，具有生长繁殖快、氮磷吸收能力强和高含蛋白质和淀粉的特性，在饲料和能源方面具有 较大的开发潜力。目前尚未有系统研究我国长江中游地区浮萍种质资源的报道。本研究从我国长江中游地区 3 个省 34 个具有 代表性的自然水体中共收集到浮萍种质 112 份。经形态学并结合分子生物学鉴定，它们分属 4 个属 6 个种，其中青萍属（Lemna) 含 3 个种 55 份种质；多根紫萍属（Spirodela)、少根紫萍属（Landoltia）、芜萍属（Wolffia)均只有 1 个种，种质数量分别 33 份、19 份和 6 份。遗传多样性分析表明它们 AtpF-AtpH 间隔序列和 rpS16 内含子序列的单倍型数量分别为 11 和 18，单倍型 多样性分别为 0.748 和 0.892，群体突变率分别为 0.03439 和 0.03507，平均每 Kb 核苷酸差异数为 31.386 和 61.263，核苷酸多 样性指数分别为 0.04713 和 0.06623。本研究为我国长江中游浮萍的资源化利用奠定了基础。

    Abstract:

    Duckweed, a family of aquatic plants, has the characteristics of fast growth and reproduction, strong nitrogen and phosphorus absorption capacity, and high protein and starch content. Therefore it has great potential in feed and bioenergy production. However, the information of duckweed germplasm collection and diversity analysis in the middle reaches of Changjiang River has not been systematically reported. In this study, 112 duckweed germplasm were collected from 34 representative natural aquatic habitats in three provinces of the middle reaches of the Changjiang River. These germplasm resources were classified into 4 genera and 6 species by morphological and molecular biological identification. Lemna contained 3 species and 34 accessions. Spirodela, Landoltia and Wolffia contained only 1 species, and their collected germplasm number was 33, 19 and 6, respectively. Genetic diversity analysis showed that the number of haplotypes of their AtpF-AtpH spacer sequences and rpS16 intron sequences were 11 and 18, the haplotype diversity was 0.748 and 0.892, the population mutation rates were 0.03439 and 0.03507, nucleotide differences per Kb were 31.386 and 61.263 and nucleotide diversity was 0.04713 and 0.06623, respectively. This study plays a key role for the utilization of duckweed in the middle reaches of the Changjiang River.

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穆瑶佳,谭德冠,于博涵,等.长江中游浮萍种质资源鉴定及遗传多样性分析[J].植物遗传资源学报,2020,21(4):938-944.

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  • 收稿日期:2019-12-05
  • 最后修改日期:2020-05-13
  • 录用日期:2020-01-19
  • 在线发布日期: 2020-07-20
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