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首页 > 过刊浏览>2020年第21卷第5期 >1230-1236. DOI:10.13430/j.cnki.jpgr.20191224003
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在小麦和叶锈菌互作过程中TCTP的表达特征
作者:
作者单位:

河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室

基金项目:

国家自然科学基金项目（31171472，31871548）；高等学校博士学科点专项科研基金资助课题（优先发展领域）（20111302130001）； 河北省应用基础研究计划重点基础研究项目（12967149D）


Expression Characteristic of Translationally Controlled Tumor Protein during the Interaction between Wheat and Puccinia Triticina
Author:
Affiliation:

Hebei Agricultural University, College of Life Sciences/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology

Fund Project:

National Natural Science Foundation of China（31171472，31871548）, Special Research Funds for Doctoral Disciplines in Universities （priority areas of development，20111302130001），Hebei Key Basic Research Project of Applied Basic Research Report（12967149D）

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    摘要:

    翻译控制肿瘤蛋白是调节植物生长发育、参与植物抵抗逆境胁迫的重要调节因子。本研究以叶锈菌生理小种 260 与 4 种含有不同抗叶锈基因的近等基因系分别组成亲和程度不同的组合为对象，采用 RT-qPCR 和 Western Blotting 方法，从 转录和翻译两个水平探讨 TCTP 在小麦与叶锈菌互作过程中的表达模式。研究结果表明，无论是转录水平还是翻译水平上， TCTP 在不亲和组合中均表现为表达量显著上调，但在不同的 Lr 基因背景下其表达模式有所不同；而在亲和组合中并未表现 出明显的变化趋势。基于 TCTP mRNA 和蛋白水平的结果，我们发现在小麦与叶锈菌互作体系中 TCTP 在转录水平和翻译水平 均受到调控，推测其在小麦抵抗叶锈菌侵染的防卫反应中可能发挥正调控作用，这将为进一步研究 TCTP 的功能及其作用机 制奠定了基础。

    Abstract:

    Translationally controlled tumor protein (TCTP) is an important regulator involved in plant growth and development as well as responses to biotic and abiotic stresses. By taking use of Puccinia triticina isolate-260 and four wheat near-isogenic lines (NILs) that form combinations with different affinity degrees, we investigated the expression of TCTP at transcription and translational levels using RT-qPCR and Western Blotting, respectively. The transcriptional level of TCTP was significantly up-regulated in the incompatible combination, but this was diverse in NILs with Lr gene. No obvious change trend in the affinity combination was observed. Gained from the results of TCTP mRNA and protein levels, the expression of TCTP was found to be modified at both transcription and translation level during the interaction between wheat and Puccinia triticina. It is speculated that it may play a positive role in the defense response of wheat against Puccinia triticina infection. This will lay the foundation for further research on the function and mechanism of TCTP.

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路兴通,麻楠,孙天杰,等.在小麦和叶锈菌互作过程中TCTP的表达特征[J].植物遗传资源学报,2020,21(5):1230-1236.

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  • 收稿日期:2019-12-24
  • 最后修改日期:2020-05-20
  • 录用日期:2020-06-08
  • 在线发布日期: 2020-09-09
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