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首页 > 过刊浏览>2020年第21卷第5期 >1214-1220. DOI:10.13430/j.cnki.jpgr.20200109002
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水稻穗发芽突变体的筛选及候选基因鉴定
作者:
作者单位:

华南师范大学生命科学学院/广东省植物发育生物工程重点实验室

基金项目:

广东省自然科学基金(2017A03013104)；福建省自然科学基金(2017J01427)


Screening and Gene Mapping of Pre-harvest Sprouting Mutants in Rice
Author:
Affiliation:

School of Life Sciences, South China Normal University/ Guangdong Provincial Key Laboratory of Biotechnology for Plant Development

Fund Project:

Natural Science Foundation of Guangdong Province(2017A03013104)；Natural Science Foundation of Fujian Province(2017J01427)

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    摘要:

    水稻穗发芽是一种多发性的自然灾害，造成严重的经济损失。由于穗发芽机制还不清楚，如何培育抗穗发芽的水 稻品种，已成为世界性的难题。本研究利用水稻 EMS（Ethylmethylsulfone）诱变突变体库，通过田间筛选，获得 9 个水稻穗 发芽突变体。并对其中两个突变体，利用基于基因组重测序的 SIMM 方法和高分辨率溶解曲线（HRM）分析技术，分别获得 其穗发芽候选基因 LOC_Os03g08570 和 LOC_Os07g10490。LOC_Os03g08570 编码一个八氢番茄红素脱氢酶（phytoene desaturase，OsPDS），LOC_Os07g10490 编码一个ζ-胡萝卜素脱氢酶（ζ-carotene desaturase，OsZDS），它们都参与 ABA 前体 类胡萝卜素的合成。本研究的结果进一步证明类胡萝卜素的合成在水稻休眠中的关键作用。同时，本研究获得的这些穗发芽 突变体为后续深入研究水稻穗发芽的分子机制及开展分子设计育种提供了宝贵材料。

    Abstract:

    Pre-harvest sprouting (PHS) is a frequently-occurring disaster that results in a serious economic loss in rice production. Breeding for PHS-resistant rice varieties has limited worldwide due to the mechanism of PHS that remains unclear. Here, nine independent mutants conferring PHS were identified by screening of an EMS (Ethylmethylsulfone) mutant library, and two of them were subjected for further study. By taking use of whole genome resequencing, SIMM method and HRM technology, two PHS mutant genes (LOC_Os03g08570 and LOC_Os07g10490) were molecularly cloned. LOC_Os03g08570 codes a phytoene desaturase (OsPDS), and LOC_Os07g10490 codes a ζ-carotene desaturase (ζ-carotene desaturase). Both were reported to be involved in the biosynthesis of carotenoid as the precursor of ABA. In summary, our data highlights the critical role of carotenoid that participates in rice dormancy. Further studies of these PHS mutants would allow revealing the mechanism of PHS and breeding for PHS-resistant rice varieties.

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倪浩凌,吴文诗,颜艳敏,等.水稻穗发芽突变体的筛选及候选基因鉴定[J].植物遗传资源学报,2020,21(5):1214-1220.

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  • 收稿日期:2020-01-09
  • 最后修改日期:2020-02-07
  • 录用日期:2020-05-15
  • 在线发布日期: 2020-09-09
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