The high temperature at the grain filling stage of spring wheat is harmful to the yield production and exploration of the quantitative loci with elevated high-temperature tolerance becomes of interest in wheat breeding. In this study, a recombinant inbred line population derived from the cross of two spring wheat lines ‘Avocet’ and ‘Sujata’ was used for the quantitative mapping of the genetic loci that associated with TKW (thousand kernel weight), KL (Kernel length) and KW (Kernel width) as well as analyzing the interaction pattern of QTLs responding to high temperature at the grain-filling stage. The field trails were conducted at four environments (Gaocheng, Shijiazhuang, 2016-2018; Tianjin, 2017). Twenty quantitative loci were detected at all of four environments, including five QTLs associated with KL, four with KW and eleven with TKW. Four loci QTkw-5A.1/QKl-5A, QTkw-2A.1/QKw-2A.2, QTkw-3B.2/QKw-3B and QTkw-6A/QKw-6A were found with double functions. Three TKW-QTLs (QTkw-2A.1, QTkw-4B and QTkw-5A.1) were detected specifically at different environments. Seven TKW-QTLs were detected at Shijiazhuang, where highand/ or super-high temperature was observed in two calendar years of 2017 and 2018. Two major QTLs (Tkw-2A.1 and QTkw-5A.1) explained 13.8% and 17.3% of phenotypic variation, respectively, whereas five minor QTLs (QTkw-2A.2, QTkw-3B.1, QTkw-3B.2, QTkw-4A.2 and QTkw-6A) contributed to 7.4%-9.9% of phenotypic variation. Out of nine TKW-QTLs detected in Shijiazhuang, six and three represented additive effects from Sujata and Avocet, respectively. Thus, the parental line ‘Sujata’ represented more elite alleles causing higher TKW and wider adaptability.