In this study, 292 sorghum breeding lines were subjected for tests against the infections of greenbug (Schizaphis graminum) and sugarcane aphid (Melanaphis sacchari) at seedling stage and adult stage, in order to identify the parental lines with reliable resistance used for resistance pyramiding in sorghum breeding. As a result, at both seedling and adult stages three genotypes including PB14648-6, PI550607 and Tx2737 represented constantly resistant against the greenbug, while the lines 3765 white B, L27 (powder), R1027 and jiya2 were resistant to sugarcane aphid. Three genotypes Hebeinuo R, Qingkeyang and Tx2737 showed moderately resistant to both greenbug and sugarcane aphid at adult stage. The statistical analysis revealed a very significant difference after 14 days infested by greenbug (p<0.01). The significant differences were observed after 7d (p<0.05) and 10d, 14d (p<0.01) infested by sugarcane aphid. The resistance varieties became visible easily, because the plants showing high-sensitive died or dried up 14 days post infection (dpi). In the identification of aphid resistance at the adult stage of sorghum ,a higher positive correlation (greenbug, 0.844; sugarcane aphid, 0.954) between the average numbers of aphids in adult plant stage, in relative to that at peak growth period was observed. The resistance level at peak growth period could be deployed to reflect that of the adult stage which could be used for the re-identification test for aphid resistance of sorghum.