With the change of the global climate， the drought tolerance at germination stage of soybean becomes of importance when suffering spring drought. In order to explore an optimal drought stress condition， four soybean accessions with levels of drought tolerance were tested with the concentrations of 0%， 10%， 15%， 16%， 18%， 20%， 30% of PEG-6000 solution simulated different drought conditions. The result showed that 15%~18% of PEG-6000 solution was qualified to simulate drought stress condition at germination stage of soybean. Moreover， 16% of PEG-6000 solution was applied to culture the seeds of 568 soybean germplasm accessions. The indexes were estimated using six indicators including relative germination energy， relative germination rate， relative radical length， relative bud length， germination drought tolerant index and vigor index. Based on subordination function method and the standard classification of drought tolerance in soybean， four drought tolerant varieties， 18 medium tolerant varieties， 110 intermediate type varieties， 194 relatively drought sensitive varieties and 242 drought sensitive varieties were identified.