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首页 > 过刊浏览>2021年第22卷第3期 >718-724. DOI:10.13430/j.cnki.jpgr.20201105002
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SSR分子标记鉴定橡胶树异源四倍体自然减倍现象
作者:
作者单位:

1.中国热带农业科学院橡胶研究所/国家橡胶树育种中心/农业农村部儋州橡胶树种质资源圃/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室;2.云南省红河热带农业科学研究所

基金项目:

国家重点研发计划（2019YFD1001105）；现代农业产业技术体系（CARS-34-YZ2）


Ploidy Reduction of Allotetraploid Hevea brasiliensis Revealed by SSR Markers
Author:
  • ZHANG Yuan-yuan 1

    ZHANG Yuan-yuan

    Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Centre for Rubber Breeding / Danzhou Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs / Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base and Hainan Provincial Key Laboratory of Cultivation Physiology of Tropical Crops
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  • ZHANG Yong 2

    ZHANG Yong

    Yunnan Honghe Institute of Tropical Agricultural Science
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  • HU Yan-shi 1

    HU Yan-shi

    Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Centre for Rubber Breeding / Danzhou Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs / Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base and Hainan Provincial Key Laboratory of Cultivation Physiology of Tropical Crops
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  • LI Wei-guo 1

    LI Wei-guo

    Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Centre for Rubber Breeding / Danzhou Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs / Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base and Hainan Provincial Key Laboratory of Cultivation Physiology of Tropical Crops
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  • HUANG Hua-sun 1

    HUANG Hua-sun

    Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Centre for Rubber Breeding / Danzhou Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs / Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base and Hainan Provincial Key Laboratory of Cultivation Physiology of Tropical Crops
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Affiliation:

1.Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences / State Centre for Rubber Breeding / Danzhou Rubber Tree Germplasm Repository, Ministry of Agriculture and Rural Affairs / Key Laboratory of Biology and Utilization of Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs / State Key Laboratory Incubation Base and Hainan Provincial Key Laboratory of Cultivation Physiology of Tropical Crops;2.Yunnan Honghe Institute of Tropical Agricultural Science

Fund Project:

National Key Research and Development Program of China (2019YFD1001105); China Agriculture Research System (CARS-34-YZ2)

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    摘要:

    植物体细胞染色体数目减少是一种罕见现象。本研究以同一份橡胶树野生种质的四倍体接穗、嵌合体接穗和嵌合体的二倍体砧木为材料，通过流式细胞术分析嵌合体中四倍体细胞比例的位置效应，利用SSR分子标记分析四倍体和嵌合体的来源途径。结果表明，嵌合体植株四倍体细胞的比例在第一蓬叶处为80.50%，在第七蓬叶位置的比例为65.58%，嵌合体中四倍体细胞比例随着植株的生长而下降。18对引物在四倍体和嵌合体中扩增出的32条带完全一致，与嵌合体的二倍体砧木中的扩增结果有28个差异条带，表明嵌合体来源于四倍体体细胞的自然减倍。18个位点中1个位点的两个等位基因相对扩增产物量为3：1，表明该四倍体种质资源不是体细胞染色体加倍形成的同源四倍体，而是由2n卵细胞和2n花粉经有性多倍化形成的异源四倍体。本研究首次发现并证实了橡胶树异源四倍体体细胞减倍现象。

    Abstract:

    The reduction of chromosome number in plant somatic cells is a rare phenomenon. In this study, the tetraploid scion, chimeric scion, and diploid rootstock of the same wild germplasm of Hevea brasiliensis were used to analyze the position effect of tetraploid cell proportion in chimeras by flow cytometry, and the origins of tetraploid and chimeric identified by SSR markers. The results showed that the proportion of tetraploid cells in the chimera plants decreased to 65.58% in the seventh leaf layer from 80.50% in the first leaf layer. The 32 bands amplified by 18 pairs of primers were identical in the tetraploid and chimera, and there were 28 different bands compared with 28 bands of the diploid rootstock, indicating that the chimera was derived from the spontaneous somatic ploidy reduction of tetraploid. The peak ratio of two alleles in one of the 18 loci was 3:1, indicating that the tetraploid germplasm was not an autotetraploid produced by chromosome doubling of somatic cells, but an allotetraploid derived from sexual polyploidization of the 2n egg and 2n pollen. This study discovered and proved the phenomenon of ploidy reduction of an allotetraploid in Hevea brasiliensis.

    参考文献
    [1]Sytnyk K, Vdovychenko Z H, Spyrydonov V, Melnychuk M, Parii M, Zimina O. Somatic segregation: genetic evidences, consequences and applications. Acta horticulturae, 2012, 961: 495-502
    [2]Rana R S. Somatic reduction in an intervarietal hybrid of chrysanthemum. The Japanese Journal of Genetics, 1965, 40:199-201.
    [3]Ronchi V N, Giorgetti L, Tonelli M, Martini G. Ploidy reduction and genome segregation in cultured carrot cell lines. I. Prophase chromosome reduction. Plant Cell, Tissue and Organ Culture, 1992, 30: 107–114.
    [4]Ronchi V N, Giorgetti L, Tonelli M, Martini G. Ploidy reduction and genome segregation in cultured carrot cell lines. II. Somatic meiosis. Plant Cell, Tissue and Organ Culture, 1992, 30: 115-119.
    [5]Giorgetti L, Vergara M R, Evangelista M, Schiavo L F, Terzi M, Ronchi V N. On the occurrence of somatic meiosis in embryogenic carrot cell cultures. Molecular and General Genetics, 1995, 246: 657–662.
    [6]顾蔚.植物激素对蚕豆胚轴愈伤组织染色体加倍和减倍的影响. 陕西师范大学学报(自然科学版),1997,27(1)：100-102.Gu W.Effect of phytohormone on chromosome polyploidization and haploidizat ion of callus of Vicia faba. Journal of Shaanxi Normal University (Natural Science Edition), 1997,27(1):100-102.
    [7]Chen Y, Zhang L, Geng Y, Chen Z. Meiosis-like reduction during somatic embryogenesis of Arabidopsis thaliana. In Vitro Cellular Developmental Biology–Plant, 2001, 37:654–657.
    [8]Naess S, Swartz H, Bauchan G. Ploidy reduction in blackberry. Euphytica, 2004, 99(1): 57-73.
    [9]Huskins L C. Chromosome multiplication and reduction in somatic tissues. Nature, 1948, 161: 80-83.
    [10]Huskins L C. Segregation and Reduction in Somatic Tissues. Journal of Heredity, 1948, 39: 311-325.
    [11]Sanders M E, Franzke C J. Somatic reduction of tetraploid Sorghum to diploid mutants following colchicine treatment. Nature, 1962, 196:696-698.
    [12]Chen Y, Zhang L, Zhou Y, Geng Y, Chen Z. Inducing somatic meiosis-like reduction at high frequency by caffeine in root-tip cells of Vicia faba. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2000, 452(1): 67-72.
    [13]张源源,濮寿琴,胡彦师,方家林,黄肖,李维国,黄华孙. 橡胶树种质资源的倍性鉴定. 热带作物学报,2020,41(3)：457-463.Zhang Y Y, Pu S Q, Hu Y S, Fang J L, Huang X, Li W G, Huang H S. Ploidy identification of germplasm resources in Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. Chinese Journal of Tropical Crops, 2020, 41(3):457-463.
    [14]http://mggm-lab.easyomics.org/search/Hevea_brasiliensis.php
    [15]Esselink G D, Nybom H, Vosman B. Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting-peak ratios) method. Theoretical and Applied Genetics, 2004, 109: 402–408.
    [16]Sharma A K, Bhattacharjee D. Somatic reduction in untreated Leguminous plants. Genetica, 1953, 26: 410–414.
    [17]Brown M S. A case of spontaneous reduction of chromosome number in somatic tissue of cotton. American Journal of Botany,1947, 34: 384-388.
    [18]Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Brub C, Kumlehn J, Matzk F, Houben A. Uniparental Chromosome Elimination at Mitosis and interphase in wheat and peral millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell, 2005, 17(9): 2431–2438.
    [19]Sanei M, Pickering R, Kumke K, Nasuda S, Houben A. Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(33): E498-E505.
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引用本文

张源源,张勇,胡彦师,等. SSR分子标记鉴定橡胶树异源四倍体自然减倍现象[J].植物遗传资源学报,2021,22(3):718-724.

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  • 收稿日期:2020-11-05
  • 最后修改日期:2021-01-04
  • 录用日期:2021-01-05
  • 在线发布日期: 2021-05-07
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