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首页 > 过刊浏览>2022年第23卷第1期 >263-271. DOI:10.13430/j.cnki.jpgr.20210624001
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蜻蜓凤梨开花相关基因AfFPF1初步研究
作者:
作者单位:

1.中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室/海南省热带作物遗传改良与创新重点实验室/国家热带作物中期库;2.南京农业大学园艺学院

基金项目:

中央级科研院所基本科研业务费专项（1630032019039）；国家自然科学基金（31372106）；海南省自然科学基金（20163127）；海南省自然科学基金(809195749021)海南省自然科学基金（20163127）；海南省自然科学基金(809195749021)


Preliminary Study of Flowering-related Gene AfFPF1 in Aechmea fasciata
Author:
  • JING Yong-lin 1

    JING Yong-lin

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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  • XUE Bing-tao 1

    XUE Bing-tao

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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  • CHEN Ying 1

    CHEN Ying

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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  • YAN Hang 1

    YAN Hang

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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  • ZHAO Ya-wen 2

    ZHAO Ya-wen

    College of Horticulture, Nanjing Agricultural University
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  • LI Zhi-ying 1

    LI Zhi-ying

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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  • XU Li 1

    XU Li

    Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops
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Affiliation:

1.Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China / Hainan Province Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation / National Gene Bank of Tropical Crops;2.College of Horticulture, Nanjing Agricultural University

Fund Project:

Special Fund for Basic Business Expenses of the Central Level Research Institutes (1630032019039)，National Natural Science Foundation of China (31372106)，Natural Science Foundation of Hainan Province (20163127)，Natural Science Foundation of Hainan Province (809195749021)

  • 摘要
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    摘要:

    FPF1 (flowering-promoting factor 1)是参与植物开花期遗传控制的重要家族之一。迄今为止，人们对蜻蜓凤梨中FPF1家族了解有限。本研究以热带花卉蜻蜓凤梨为材料，基于转录组测序数据，克隆获得AfFPF1基因。该基因编码的蛋白含103个氨基酸，分子质量为12.06 kD。AfFPF1转录本在蜻蜓凤梨的根显著高表达，此外其表达量受外源乙烯强诱导，且响应赤霉素。AfFPF1基因在拟南芥中异位表达使其开花时间提前，莲座叶数目减少，促进其根系生长。对转基因拟南芥内源开花基因进行表达量检测，发现转基因植株中，一些开花促进基因如AtFT、AtAP1、AtLFY、AtSOC1表达量显著升高，而抑制开花基因AtFLC表达量下调，进一步证实AfFPF1能正调控拟南芥的开花时间，且可能与这些基因有相互作用。研究结果初步证实 AfFPF1可能参与调控蜻蜓凤梨开花过程，为进一步研究AfFPF1功能、通过基因工程手段调控蜻蜓凤梨开花以及乙烯诱导蜻蜓凤梨开花分子机制提供了理论依据。

    Abstract:

    FPF1 (flowering-promoting factor 1) is one of the important family involved in the genetic control of flowering time in plant. So far, the knowledge concerning the FPF1 family in Aechmea fasciata has been very much limited. In this study, the AfFPF1 gene was cloned from A. fasciata, a tropical flower, based on the transcriptome sequencing data. The protein encoded by this gene contained 103 amino acids and had a molecular mass of 12.06 kD. The AfFPF1 gene was ectopically expressed in Arabidopsis thaliana, which advanced the flowering time of A. thaliana, reduced the number of rosette leaves and promoted the growth of root system. Some flowering-promoting genes such as AtFT, AtAP1, AtLFY and AtSOC1 were found to be significantly increased in the transgenic plants, while the expression of the flowering-inhibiting gene AtFLC was down-regulated. This further confirmed that AfFPF1 could positively regulate the flowering time in A. thaliana and might interact with these genes. These results demonstrated that AfFPF1 might be involved in regulating the flowering process of Aechmea fasciata, providing a theoretical basis of characterization of AfFPF1, the regulation of A. fasciata flowering using genetic approach and the molecular mechanism of ethylene-induced flowering in the near future.

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荆永琳,薛丙涛,陈莹,等.蜻蜓凤梨开花相关基因AfFPF1初步研究[J].植物遗传资源学报,2022,23(1):263-271.

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  • 收稿日期:2021-06-24
  • 最后修改日期:2021-07-22
  • 录用日期:2021-08-11
  • 在线发布日期: 2022-01-07
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