158 份春兰种质资源的表型多样性分析
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1.江苏里下河地区农业科学研究所;2.江苏省农业技术推广总站

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基金项目:

江苏现代农业产业技术体系建设专项资金(JATS[2021]264);江苏省农业种质资源保护与利用平台;扬州市现代农业发展项目 (扬农[2021]74 号);江苏里下河地区农科所科研专项基金资助项目(SJ(21)302)


Phenotypic Diversity Analysis of 158 Cymbidium goeringii Germplasm Resources
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1.Institute of Agricultural Sciences of Lixiahe Districts;2.Jiangsu Agricultural Technical Extension Station

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Jiangsu Agricultural Industry Technology System(JATS[2021]264),Jiangsu Province Agricultural Germplasm Resources Protection and Utilization Platform,Yangzhou Modern Agriculture Development Project(Yangnong[2021]74),Institute of Agricultural Sciences of Lixiahe Districts Scientific Research Fund Project(SJ(21)302)

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    摘要:

    以 158 份不同地区的春兰种质资源为参试材料,通过方差分析、遗传变异系数、多样性指数分析、主成分分析、聚类 分析和相关性分析,对 20 个表型性状进行多样性分析。结果显示:20 个表型性状在春兰群体内存在丰富的变异,数量性状花 葶高和侧瓣宽的变异系数最大,为 36.96%,叶长和叶宽的多样性指数最大,均为 7.28;质量性状瓣型变异系数(66.32%)最高, 花瓣色变异系数(60.47%)次之,外瓣紧边的多样性指数最高,为 7.28。主成分分析结果显示,舌面红点、苞筋色、花葶色等性 状是春兰表型变异的主要因素,并通过综合得分(F 值)获得排名前 10 的品种:天彭牡丹、笑飞燕、碧瑶、杜字、新春梅、万年 梅、宪荷、天兴梅、紫金梅和环球荷鼎。系统聚类将春兰种质分为 3 类,类群Ⅰ为中株型、有色花、花瓣拱抱状材料,类群Ⅱ为高 株型、素花材料,类群Ⅲ为矮株型、长叶、有色花且花瓣翻卷材料。本研究为春兰种质资源的表型多样性鉴定分析提供重要数 据,为兰属品种选育及种间杂交遗传多样性研究提供理论基础。

    Abstract:

    158 germplasm accessions of Cymbidium goeringii (Rchb. f.) Rchb. f. from different regions were used to analyze the diversity of 20 phenotypic traits,followed by the analysis of variance,coefficient of variation analysis,Shannon-wienter index analysis,principal component analysis,cluster analysis and correlation analysis. Abundant variations on 20 phenotypic traits were observed in Cymbidium goeringii(Rchb. f.) Rchb. f. population. The coefficient of variation on the scape height and the side petal width was the largest (36.96%). The diversity index of leaf length and leaf width was the highest (both 7.28). The coefficient of variation on the petal type (66.32%) was the highest,followed by that of petal color (60.47%). The diversity index of arch edge outside petal was the highest (7.28). The principal component analysis showed that three traits including the red spot on lip,the bract tendon color,and scape color were the main factors that largely contributed to the phenotypic variation. By applying with the comprehensive score (F value),ten varieties were identified (Tian peng mu dan,Xiao fei yan,Bi yao,Du zi,Xin chun mei,Wan nian mei,Xian he,Tian xing mei,Zi jin mei and Huan qiu he ding). These germplasm resources were divided into three groups by systematic clustering anlysis. Of them,Group-Ⅰ was medium plant type,colored flower and circle petal material,group-Ⅱ was tall plant type and pure color material,and group-Ⅲ was dwarf plant type,long leaf,colored flower and rolled petalmaterial.Altogether,this study provided insights on deciphering the phenotypic diversity of Cymbidium Sw. germplasm resources,valuable as a theoretical basis in breeding of orchid varieties.

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引用本文

魏晓羽,刘 红,瞿 辉,等.158 份春兰种质资源的表型多样性分析[J].植物遗传资源学报,2022,23(2):398-411.

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  • 收稿日期:2021-10-27
  • 最后修改日期:2021-12-02
  • 录用日期:2021-12-17
  • 在线发布日期: 2022-03-09
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