1.山东师范大学生命科学学院;2.山东省农科院经济作物研究所;3.山东师范大学生命科学学院;4.山东省农科院经济作物研究所
国家现代农业产业技术体系(CARS-15-05);山东省农业良种工程(2020LZGC002)
1.College of Life Science,Shandong Normal University;2.Institute of Industrial Crops,Shandong Academy of Agricultural Sciences
National Project of Modern Agricultural Industry Technology System in China(CARS-15-05),Agricultural Breeding Improvement Project of Shandong Province (2020LZGC002)
早熟性状是棉花遗传改良的目标性状之一,鉴定棉花早熟性状 QTL 具有重要育种价值。以陆地棉早熟品系 EM019(Early-maturity 019)和陆地棉晚熟品种鲁棉研 37 号(LMY37,Lumianyan 37)为亲本杂交,构建了包含 312 个株系的 重组自交系群体(RILs,recombinant inbred lines),采用复合区间作图法对该 RIL 群体在山东临清(2019,2020,2021 年)、新 疆轮台(2019 年)4 个环境条件下的开花时间、吐絮率、铃重、衣分进行 QTL 检测,共获得 21 个 QTLs。其中 7 个与开花时间 相关,加性效应在 0.30~1.01 之间,解释表型变异率为 2.56%~17.71%;8 个与吐絮率相关,加性效应在 -5.81~-2.39 之间,解释 的表型变异率为 4.66%~20.44%。这些 QTLs 大多成簇分布在 A05、D03 和 D08 染色体上。对 D08 染色体上的一个 QTL 簇 进行相关基因鉴定,筛选出 124 个相关基因,结合 LMY37 和 EM019 在长日照和短日照处理下不同叶龄期的转录组数据,通 过 GO 富集和热图分析,获得了 3 个可能与早熟相关的基因:GH_D08G0636、GH_D08G0684 和 GH_D08G0948。本研究为后 续棉花早熟相关性状 QTL 的精细定位和相关基因鉴定奠定了理论基础。
Early maturity is one of the target traits in upland cotton(Gossypium hirsutum L.)breeding,and identification of QTL related to early-maturity is of practical value. In our study,we crossed early-maturity 019 line(EM019)with a late-maturing cultivar Lumianyan 37(LMY37),followed by continuous self-pollination and construction of a recombinant inbred line(RIL)population composed of 312 lines. The RIL population was planted in Linqing(2019,2020 and 2021)and Xinjiang(2019). We used composite interval mapping (CIM)method to detect quantitative trait locus(QTLs)underlying the flowering time(FT),percentage of open bolls(POB),boll weight(BW)and lint percentage(LP). A total of twenty-one early-maturity QTL had been identified,and seven QTL were detected with the additive effect ranging from 0.3 to 1.01,explaining 2.56%~17.71% of the phenotypic variation. Eight QTL associating with POB were identified with the additive effect of 5.81~-2.39,explaining 4.66%~20.44% of the phenotypic variation. These QTL were mainly distributed on Chromosomes A05,D03 andD08. A QTL cluster was found on Chromosome D08,in which 124 candidate genes were annotated based on the transcriptome data of LMY37 and EM019 at different true leaf stages under long and short day treatments. Three strong related genes namely GH_D08G0636,GH_D08G0684 and GH_ D08G0948,have been identified. Altogether,these QTL identified from this study provided insight for future deciphering the molecular mechanisms of early-maturity related traits in cotton.
吕婉玉,高 阳,?,等.陆地棉早熟相关性状的QTL定位与相关基因的初步鉴定[J].植物遗传资源学报,2022,23(3):800-810.
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