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首页 > 过刊浏览>2022年第23卷第3期 >895-905. DOI:10.13430/j.cnki.jpgr.20211209002
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苦荞转录因子FtMYB41的克隆及功能分析
作者:
作者单位:

1.长江大学农学院;2.中国农业科学院作物科学研究所

基金项目:

国家重点研发计划（2019YFD1000700，2019YFD1000703）


Molecular Cloning and Functional Analysis of Transcription Factor FtMYB41 in Tartary Buckwheat（Fagopyrum Tataricum）
Author:
Affiliation:

1.College of Agriculture,Yangtze University,Hubei Jingzhou;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences

Fund Project:

The National Key Rssearch and Development Program of China（2019YFD1000700，2019YFD1000703）

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    摘要:

    本试验以品苦一号为材料，克隆得到 FtMYB41 基因。基因结构分析表明：FtMYB41 由 1 个 5′UTR 和 2 个内含子 构成，CDS 全长 705bp，编码 234 个氨基酸序列，属于 MYB（Myeloblas-toma）超级家族蛋白。系统进化树分析表明：FtMYB41 氨基酸编码蛋白与番茄 NP_001234262.1 和烟草 NP_001312384.1 同源关系较近。转录激活活性分析表明：FtMYB41 具有转 录激活活性并且转录激活区域在羧基端（C 端）。qRT-PCR 结果分析显示：FtMYB41 基因在根中表达量最高，并且受到干旱 和盐胁迫的诱导表达，干旱胁迫 9 h 表达量显著提高。过表达拟南芥和苦荞毛状根抗逆性鉴定表明：FtMYB41 基因的过表达 通过提高发芽率，增加过氧化氢酶（CAT，catalase）和超氧化物歧化酶（SOD，superoxide dismutase）酶活，降低丙二醛（MDA， malondialdehyde）含量来提高抗旱性。

    Abstract:

    In this study，we cloned the transcription factor gene FtMYB41 in tartary buckwheat cultivar Pinku-1. Sequence analysis showed that the MYB super gene family member FtMYB41 contained one 5′UTR and two introns，with the complete coding sequence of 705bp which encodes for 234 amino acids. Phylogenetic tree analysis showed that FtMYB41 protein had close homology with tomato NP_001234262.1 and tobacco NP_001312384.1. Transcriptional activation analysis revealed the transcriptional activation activity at FtMYB41 C-terminal. Moreover，the FtMYB41 gene had the higher expression in roots and was induced by drought and salt stresses. The expression level of FtMYB41 gene was significantly increased under drought stress for 9 h. Overexpressing FtMYB41 gene in Arabidopsis thaliana（L.）Heynh. and tartary buckwheat hair root resulted in improved drought resistance by increasing germination rate，increasing CAT and SOD activity，and decreasing MDA content.

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康 珍,杨 迪,郝彦蓉,等.苦荞转录因子FtMYB41的克隆及功能分析[J].植物遗传资源学报,2022,23(3):895-905.

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  • 收稿日期:2021-12-09
  • 最后修改日期:2021-12-17
  • 录用日期:2021-12-30
  • 在线发布日期: 2022-05-11
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