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首页 > 过刊浏览>2022年第23卷第3期 >787-799. DOI:10.13430/j.cnki.jpgr.20211218001
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大豆 DELLA 基因单倍型与花期关联分析及基因编辑靶点鉴定
作者:
作者单位:

广州大学生命科学学院

基金项目:

国家自然科学基金项目（32072013）


Association Analysis of Soybean DELLA Gene Haplotypes with Flowering Time and Identification of Their Gene-editing Target Sites
Author:
Affiliation:

School of Life Sciences,Guangzhou University

Fund Project:

National Natural Science Foundation of China (32072013)

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    摘要:

    DELLA 蛋白作为赤霉素负调控因子参与植物开花期调控。与拟南芥 DELLA 蛋白同源比对，研究发现大豆具有 8 个 DELLA 基因，其中 GmGAI3a 只有 GRAS 结构域，其他 7 个 DELLA 蛋白均具有 DELLA 结构域和 GRAS 结构域。对 8 个 大豆 DELLA 基因在 424 份材料的自然群体中的单倍型与开花期进行关联分析，发现 DELLA 基因早花型自然变异已经应用 于我国中高纬度地区，推测大豆 DELLA 基因负向调控开花。利用 CRISPR/Cas9 技术，在大豆发根系统中验证靶点的编辑效 率，成功鉴定出 7 个 DELLA 基因的有效靶点，为进一步获得稳定遗传转化的大豆 DELLA 突变体及解析基因功能提供了重要 的靶点信息与理论基础。

    Abstract:

    DELLA proteins are known as negative regulators of gibberellin involved in plant flowering. Eight DELLA genes were identified in soybean genome by sequence alignment with Arabidopsis thaliana（L.） Heynh. DELLA orthologs GmGAI3a has only one GRAS domain，and the other seven DELLA proteins have both DELLA domain and GRAS domain. By performing gene-based association analysis of flowering time in natural population，soybean DELLA haplotypes associating with early-flowering have been detected in soybean accessions collected from the middle and high latitude of China，speculating DELLA genes as negative factors in regulating flowering. CRISPR/Cas9-based editing in soybean hairy root system revealed the editing efficiency at the target sites. Identification of the CRISPR/Cas9 targets of seven DELLA genes provided references for generating stable transgenic DELLA mutants for deciphering their biological functions.

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贺米兰,李海洋,黄泽荣,等.大豆 DELLA 基因单倍型与花期关联分析及基因编辑靶点鉴定[J].植物遗传资源学报,2022,23(3):787-799.

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  • 收稿日期:2021-12-18
  • 最后修改日期:2022-01-07
  • 录用日期:2022-02-07
  • 在线发布日期: 2022-05-10
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