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首页 > 过刊浏览>2022年第23卷第5期 >1446-1457. DOI:10.13430/j.cnki.jpgr.20220305001
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基于分离世代和RIL群体的亚麻株高遗传定位
作者:
作者单位:

甘肃省农业科学院作物研究所

基金项目:

国家特色油料产业技术体系(CARS-14-1-05)；甘肃省农业科学院农业科技创新专项(2019GAAS32；2020GAAS08)；甘肃省知识产权计划项目(21ZSCQ026)；兰州市科技计划项目(2021-1-35; 2021-RC-64)


Genetic Analysis of Plant Height in Flax Using Segregating Generations and Recombination Inbred Line Populations
Author:
Affiliation:

Crop Research Institute,Gansu Academy of Agricultural Sciences

Fund Project:

National Characteristic Oil Industry Technology System(CARS-14-1-05)，Agricultural Science and Technology Innovation of Gansu Academy of Agricultural Sciences(2019GAAS32; 2020GAAS08)，Gansu Intellectual Property Project(21ZSCQ026)，Lanzhou Science and Technology Project(2021-1-35; 2021-RC-64)

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    摘要:

    株高是亚麻主要的株型性状，与亚麻的抗倒性、产量等密切相关。为深入研究亚麻株高的遗传规律，本研究利用株高具有显著差异的亲本组合“P.I.250089×黑亚15号”构建分离世代，采用植物数量性状主基因+多基因混合遗传分离分析方法研究亚麻株高的遗传模型，同时以RIL群体为材料基于二代测序技术的BSA性状定位方法，对亚麻株高进行基因定位。研究结果表明，亚麻株高符合2对加性-显性-上位性主基因+加性-显性-上位性多基因遗传模型， B1、B2和F2世代主基因遗传率分别为7.68 %、0.0 %和13.86 %，多基因遗传率分别为70.88 %、0.0 %和0.0 %，亚麻株高遗传以两对主基因的上位性效应为主，其次为加性效应和显性效应。通过BSA定位分析，在第14条染色体5,770,000 bp～6,100,000 bp和6,920,000 bp～9,400,000 bp共发现2个候选基因位点，区间大小分别为0.33 Mb和2.48 Mb，含有176个候选基因。该研究结果对于揭示亚麻株高遗传机制及指导亚麻株型改良具有重要意义。

    Abstract:

    Plant height as the main plant architecture trait is closely related with lodging resistance and yield in flax. In order to analyze the genetic mechanism of plant height, in this study, several populations derived from the cross of P.I.250089×Heiya15 were constructed, followed by the genetic analysis using the method of mixed inheritance separation analysis of major gene plus polygene of plant quantitative traits. The quantitative trait (QTL) mapping of flax plant height was performed using RIL population together with BSA method based on the next-generation sequencing technology. The results showed that the plant height of flax was controlled by two pairs of major genes with additive-dominate-epistatic effects plus poly-genes with additive-dominate-epistatic effects. The heritability of the major genes in B1, B2 and F2 generations was 7.68%, 0.0% and 13.86%, and that of polygenes was 70.88%, 0.0% and 0.0% respectively. The inheritance of flax plant height is dominated by epistatic effect of two major genes, followed by additive effect and dominant effect. QTL mapping revealed two candidate gene loci which were on chromosome 14, 5770000 bp-6100000 bp and 6920000 bp-9400000 bp, with interval sizes of 0.33 Mb and 2.48 Mb, respectively, harboring 176 candidate genes. Collectively, our results provided insight for revealing the genetic mechanism of plant height in flax breeding.

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王利民,党 照,赵 玮,等.基于分离世代和RIL群体的亚麻株高遗传定位[J].植物遗传资源学报,2022,23(5):1446-1457.

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  • 收稿日期:2022-03-05
  • 最后修改日期:2022-04-15
  • 录用日期:2022-04-22
  • 在线发布日期: 2022-09-09
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