1.重庆市农业科学院;2.重庆市农村土地整治中心
重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0541);现代农业产业技术体系专项资金资助(CARS-23-A08);重庆市农业科学院绩效激励引导专项(cqaas2019jxjl01)
1.Vegetable Research Institute, Chongqing Academy of Agricultural Sciences;2.Chongqing Rural Land Improvement Center
the Natural Science Foundation of Chongqing (cstc2019jcyj-msxmX0541),earmarked fund for China Agriculture Research System (CARS-23-A08),earmarked fund for performance incentive and guidance of Chongqing Academy of Agricultural Sciences (cqaas2019jxjl01)
为了探索单性结实分子调控网络,准确获得控制单性结实性状的基因,为茄子单性结实育种提供基础材料,本研究构建了茄子单性结实/非单性结实性近等基因系。试验采用杂交、回交和自交技术,结合单性结实鉴定和农艺性状调查,获得两组近等基因系JDX1和JDX8。利用覆盖茄子基因组的219对SSR引物对茄子近等基因系及亲本进行遗传背景分析。结果表明,近等基因系JDX1和JDX8与轮回亲本的遗传背景相似度分别达97.14%和88.23%,说明两组近等基因系材料遗传背景与其轮回亲本基本一致。单性结实鉴定结果表明,与轮回亲本比,近等基因系JDX1与JDX8已经完全转育成非单性结实株系。对两组单性结实近等基因系农艺性状调查结果表明,轮回亲本相比,JDX1和JDX8株高、首花高度、节间距、叶片长和宽、果实长和粗均不存在显著差异。结合果色、株型等农艺性状,可以得出单性结实近等基因系JDX1和JDX8的表型基本与轮回亲本一致的结论。该材料的成功创制为单性结实基础研究奠定良好的材料基础。
To illustrate the regulation mechanism of parthenocarpy and the genes controlling this process, as well as provide basic materials in eggplant parthenocarpy breeding, the parthenocarpy/non parthenocarpy near isogenic lines were constructed in this study. Two groups of near isogenic lines (NILs) JDX1 and JDX8 were generated by hybridization, backcross and selfing, in combination with parthenocarpy identification and agronomic traits investigation. 219 pairs of SSR primers covering eggplant genome were used to genotype eggplant NILs and their parents. JDX1 and JDX8 showed 97.14% and 88.23% on the genetic similarity with respective reincarnation parents, respectively. The parthenocarpy identification showed that, in difference with recurrent parents, JDX1 and JDX8 had been completely transformed into non parthenocarpic lines. There were no significant differences in plant height, first flower height, node spacing, leaf length and width, fruit length and thickness between NILs and their recurrent parents. By considering the agronomic characteristics such as fruit color and strain type, JDX1 and JDX8 showed higher similarity/identity with the reincarnation parent. Collectively, the success on creation of eggplant parthenocarpy lines laid a foundation for the basic research of single solidity.
周珊珊,杨 洋,唐晓华,等.茄子单性结实近等基因系的创建及遗传背景分析[J].植物遗传资源学报,2022,23(5):1343-1351.
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