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首页 > 过刊浏览>2023年第24卷第1期 >32-43. DOI:10.13430/j.cnki.jpgr.20220602001 优先出版
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种质圃作物种质资源安全保存策略与实践
DOI:
10.13430/j.cnki.jpgr.20220602001
CSTR:
作者:
  • 卢新雄 1

    卢新雄

    中国农业科学院作物科学研究所, 北京 100081
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  • 王力荣 2

    王力荣

    中国农业科学院郑州果树研究所,郑州 450009
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  • 辛霞 1

    辛霞

    中国农业科学院作物科学研究所, 北京 100081
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  • 尹广鹍 1

    尹广鹍

    中国农业科学院作物科学研究所, 北京 100081
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  • 张金梅 1

    张金梅

    中国农业科学院作物科学研究所, 北京 100081
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  • 陈晓玲 1

    陈晓玲

    中国农业科学院作物科学研究所, 北京 100081
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  • 何娟娟 1

    何娟娟

    中国农业科学院作物科学研究所, 北京 100081
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  • 刘运霞 1

    刘运霞

    中国农业科学院作物科学研究所, 北京 100081
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作者单位:

1.中国农业科学院作物科学研究所, 北京 100081;2.中国农业科学院郑州果树研究所,郑州 450009

作者简介:

研究方向为作物种质资源保存,E-mail: luxinxiong@caas.cn

通讯作者:

中图分类号:

基金项目:

中国农业科学院科技创新工程


Strategy and Practice of the Safe Conservation of Crop Germplasm Resources in National Field Genebanks of China
Author:
  • LU Xin-xiong 1

    LU Xin-xiong

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • WANG Li-rong 2

    WANG Li-rong

    Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
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  • XIN Xia 1

    XIN Xia

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • YIN Guang-kun 1

    YIN Guang-kun

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • ZHANG Jin-mei 1

    ZHANG Jin-mei

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • CHEN Xiao-ling 1

    CHEN Xiao-ling

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • HE Juan-juan 1

    HE Juan-juan

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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  • LIU Yun-xia 1

    LIU Yun-xia

    Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
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Affiliation:

1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;2.Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009

Fund Project:

Foundation project: Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences

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    摘要:

    苹果、梨、柑橘、香蕉等果树作物,马铃薯、甘薯、木薯等薯类作物,以及橡胶、椰子、油棕等热带经济作物,需通过植株、块根、块茎、鳞茎、种茎等繁殖体在种质圃进行种植保存,这类作物统称为“种质圃保存作物”或“圃位保存作物”。近40年以来,通过建设良好保存设施,构建规范化保存技术和实施资源复份保存,创建了种质圃作物种质资源安全保存策略。随着国家作物种质库新库的试管苗和超低温保存设施的投入使用,我国初步建成了以种质圃为主,集种质圃、试管苗库、超低温库、原生境保护点为一体的保护设施体系,为实现圃位作物种质资源多样性、完整性、安全性的保存和持续利用奠定了可靠保障。至2021年12月,我国43个国家种质圃共收集保存种质资源69,504份,隶属1,469个物种(含亚种),是世界上种质圃保存资源数量最多的国家。本文阐述了种质圃保存作物的繁殖特性,植株安全保存影响因素与技术策略,实践应用及展望等,以期对我国种质圃作物种质资源的安全保存起到促进作用。

    关键词:作物种质资源;种质圃;保存策略;安全保存
    Abstract:

    The crops, including apple, pear, citrus, banana and other fruit tree crops, and potato, sweet potato, cassava and other tuber crops, as well as rubber, coconut, oil palm and other tropical economic crops, have to be planted through propagules such as plants, roots, tubers, bulbs and seed stems. Based on the difference on long-term conservation strategy, these species are referred “field genebank conservation crops”. In the past 40 years, through the construction of conservation facilities, technical procedures on sampling and preservation, as well as backup preservation, the strategy of safe conservation of germplasm resources in germplasm field has been created in National Field Genebanks of China. With the application of newly-established in vitro and cryopreservation facilities in National Crop Genebank of China, a preliminary integrated conservation system, including the National Field Genebanks as the core, in vitro genebank, cryopreservation genebank and in situ protection sites, is able to guarantee the long-term safe conservation and continuous utilization of diversified field genebank preservation crops. By December of 2021, forty-three National Field Genebanks have maintained 69,504 accessions of 1,469 species or subspecies, hosting the largest number of accessions in the world. This article reviews the reproductive characteristics of the conserved germplasm resources in National Field Genebanks of China, the factors that affect safe conservation and the corresponding strategies that address to these factors, as well as the practical applications and prospects of the conservation facilities system, in order to provide insights on safe preservation of these special germplasm resources in China.

    Key words:crop germplasm resources;field genebank;conservation strategy;safe conservation
    参考文献
    [1] Saad M S, Rao V R. Establishment and management of field genebank, a training manual. Serdang:IPGRI-APO,2001:59-63
    [2] Postman J, Oliphant J, Hummer K. Diseases impact management of USDA clonal Vaccinium genebank. Acta Horticulturae, 2008, 810: 319-324
    [3] 王力荣. 我国果树种质资源科技基础性工作30年回顾与发展建议. 植物遗传资源学报, 2012, 13(3): 343-349Wang L R. Review for the past three decades and developmental suggestions for fruit germplasm resource in China. Journal of Plant Genetic Resources, 2012, 13(3): 343-349
    [4] Sun M. The mystery of Florida’s citrus canker. Science, 1984, 226: 322-323
    [5] 许英, 陈建华, 栾明宝, 王晓飞, 孙志民. 苎麻种质资源保存技术研究进展. 植物遗传资源学报, 2011, 12(2): 184-189Xu Y, Chen J H, Luan M B, Wang X F, Sun Z M. Research progress on conservation techniques for ramie germplasm resources. Journal of Plant Genetic Resources, 2011, 12(2): 184-189
    [6] 杨勇, 赵红星, 李高潮, 阮小凤, 王仁梓. 柿种质资源田间耐寒性调查分析. 北方园艺, 2010(1): 63-65Yang Y, Zhao H X, Li G C, Ruan X F, Wang R Z. Field investigation of cold tolerance in persimmon (Diospyros L.) germplasm. Northern Horticulture, 2010(1): 63-65
    [7] 张雄坚, 房伯平, 陈景益, 安康. 甘薯资源温室盆栽保存探讨. 广东农业科学, 2002(3): 11-14Zhang X J, Fang B P, Chen J Y, An K. Study on the pot culturing for preservation of sweet tomato resources in greenhouse. Guangdong Agricultural Sciences, 2002(3): 11-14
    [8] 贾敬贤, 贾定贤, 任庆棉. 中国作物及其野生近缘植物—果树卷. 北京: 中国农业出版社, 2006: 30-39Jia J X, Jia D X, Ren Q M. Crops and their wild relatives in China-vol. fruit tree. Beijing: China Agriculture Press, 2006: 30-39
    [9] Hummer K E, Postman J D, Preece J E. Managing nut genetic resources under disease threat. Acta Horticulturae, 2015 (1070): 193-200
    [10] Postman J, Hummer K, Stover E, Krueger R, Forsline P, Grauke L J, Zee F, Ayala-silva T, Irish B. Fruit and nut genebanks in the US National Plant Germplasm System. Hortscience, 2006, 41(5): 1188-1194
    [11] Rosales F E, Belalcázar C S, Pocasangre L E. Global conservation strategy for musa (banana and plantain). Rome: Bioversity International, 2006
    [12] Wyatt R. Pollinator-plant interactions and the evolution of breeding systems//Real L. Pollination biology. Orlando: Academy Press, 1983: 51-95
    [13] 王洪新, 胡志昂. 植物的繁育系统、遗传结构和遗传多样性保护. 生物多样性, 1996, 4(2): 92-96Wang H X, Hu Z A. Plant breeding system, genetic structure and conservation of genetic diversity. Biodiversity Science, 1996,4(2):92-96
    [14] 王崇云. 植物交配系统与遗传资源的保护和持续利用.云南大学学报:自然科学版, 1997(S1): 93-96Wang C Y. Plant mating systems and conservation and sustainable use of genetic resources. Journal of Yunnan University:Natural Science Edition, 1997(S1): 93-96
    [15] Yang H, Wei C L, Liu H W, Wu J L, Li Z G, Zhang L, Jian J B, Li Y Y, Tai Y L, Zhang J, Zhang Z Z, Jiang C J, Xia T, Wan X C. Genetic divergence between Camellia sinensis and its wild relatives revealed via genome-wide SNPs from RAD sequencing. PLoS ONE, 2016, 11(3): e0151424
    [16] Feurtey A, Cornille A, Shykoff J A, Snirc A, Giraud T. Crop-to-wild gene flow and its fitness consequences for a wild fruit tree: Towards a comprehensive conservation strategy of the wild apple in Europe. Evolutionary Applications, 2017, 10: 180-188
    [17] 陈元桥, 陈全, 刘卓慧, 李强, 白殿一, 吴晶, 王凤泰, 姜铁白. GB-T28001-2019, 职业健康安全管理体系-规范. 北京: 中国标准出版社, 2020Chen Y Q, Chen Q, Liu Z H, Li Q, Bai D Y, Wu J, Wang F T, Jiang T B. GB/T28001-2019, Occupational health and safety management system—specification. Beijing: Standards Press of China, 2020
    [18] 卢新雄, 辛霞, 刘旭. 作物种质资源安全保存原理与技术.北京:科学出版社, 2019: 195-228Lu X X, Xin X, Liu X. The principle and technology of safe conservation of crop germplasm resources. Beijing: Science Press, 2019: 195-228
    [19] 辛霞,尹广鹍,张金梅,陈晓玲,何娟娟,刘运霞,黄雪琦,卢新雄. 作物种质资源整体保护策略与实践. 植物遗传资源学报2022,23(3): 636-643Xin X, Yin G K, Zhang J M, Chen X L, He J J, Liu Y X, Huang X Q, Lu X X. Strategies and practices of the integrated conservation system for crop germplasm resources. Journal of Plant Genetic Resources, 2022, 23(3):636-643
    [20] 卢新雄, 陈叔平, 刘旭. 农作物种质资源保存技术规程. 北京: 中国农业出版社, 2008: 28-39Lu X X, Chen S P, Liu X. Technical regulation on conservation for crop germplasm resources. Beijing: China Agriculture Press, 2008: 28-39
    [21] 师文贵, 李志勇, 卢新雄, 李鸿雁, 刘磊. 国家多年生牧草种质圃资源保存规程. 中国草地学报, 2009, 31(6): 109-112Shi W G, Li Z Y, Lu X X, Li H Y, Liu L. Regulations of conservation of resources in national perennial forage germplasm nursery. Chinese Journal of Grassland, 2009, 31(6): 109-112
    [22] 孙玉芳, 雷波, 张宏斌, 卢琳琳, 李垚奎, 赵欣. 我国农业野生植物资源原生境保护区建设现状、问题及未来的思考. 中国农业资源与区划, 2016, 37(6): 224-228Sun Y F, Lei B, Zhang H B, Lu L L, Li Y K, Zhao X. In-situ conservation site of agricultural wild plants in China discussion on present situation and the way-out. Chinese Journal of Agricultural Resources and Regional Planning, 2016, 37(6): 224-228
    [23] FAO. Genebank standards for plant genetic resources for food and agriculture. Rome: FAO, 2014: 65-114
    [24] Maghradze D, Maletic E, Maul E, Faltus M, Faillas O. Field genebank standards for grapevines (Vitis vinifera L.). Vitis: Journal of Grapevine Research, 2015, 54: 273-279
    [25] 王述民,卢新雄,李立会. 作物种质资源繁殖更新技术规程. 北京: 中国农业科学技术出版社, 2014Wang S M, Lu X X, Li L H. The technical regulation on regeneration of crop germplasm resources.Beijing: China Agricultural Science and Technology Press, 2014
    [26] 白晓燕, 王力荣, 朱更瑞, 王新卫, 曹珂, 方伟超, 陈昌文, 李勇. 桃砧木半硬枝扦插繁殖影响因子的研究. 中国果树, 2014 (5):36-39Bai X Y, Wang L R, Zhu G R, Wang X W, Cao K, Fang W C, Chen C W, Li Y. Study on influencing factors of semi-hard branch cutting propagation of peach rootstock. China Fruit, 2014 (5):36-39
    [27] 李勇, 方伟超, 朱更瑞, 王力荣, 彭福田. 双容器与控根器限根对桃树生长发育的影响. 果树学报, 2014, 31(2): 213-220Li Y, Fang W C, Zhu G R, Wang L R, Peng F T. Effect of rooting-zone restriction with pot-in-pot and root-control device on the growth and development of peach. Journal of Fruit Science, 2014, 31(2): 213-220
    [28] Ni?o D P, Aranzales Rondón E, Erazo J C, López J J, Vélez M. The bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT. Poster presented at the II Symposium of Neotropical Plant Genetic Resources Valle del Cauca Colombia: National University of Colombia Palmira Headquarters, 2016
    [29] Reed B M, Engelmann F, Dulloo M E, Engels J M M. Technical guidelines for the management of field and in vitro germplasm collections. Rome: International Plant Genetic Resources Institute, 2004
    [30] Panis B, Swennen R, Piette B, Van Den Houwe I. Cryopreservation of the banana germplasm collection at the international transit centre-biodiversity international. Advances in Horticultural Science, 2007, 21(4): 1000-1004
    [31] Reed B M, Hummer K E. Cryopreservation and maintenance of hop material in the USDA germplasm collection. Acta Horticulturae, 2013, 1010: 191-197
    [32] Vollmer R, Villagaray R, Egusquiza V, Espirilla J, Garcia M, Torre A, Rojas E, Panta A, Barkley N A, Ellis D. The potato cryobank at the international potato center (CIP): A model for long term conservation of clonal plant genetic resources collections of the future. Cryo Letters, 2016, 37(5): 318-329
    [33] Jenderek M M, Reed B M. Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System. In Vitro Cellular & Developmental Biology-Plant, 2017, 53(4): 299-308
    [34] Zhang J M, Han L, Lu X X, Volk G M, Xin X, Yin G K, He J J, Wang L, Chen X L. Cryopreservation of Jerusalem artichoke cultivars using an improved droplet-vitrification method. Plant Cell, Tissue and Organ Culture, 2017a, 128: 577-587
    [35] Zhang J M, Lu X X, Xin X, Yin G K, He J J, Huang B, Jiang D, Chen X L. Cryopreservation of citrus anthers in the National Crop GeneBank of China. In Vitro Cellular and Developmental Biology-Plant, 2017b, 51(4): 445-451
    [36] 张金梅, 闫文君, 李雪, 黄斌, 张海晶, 赵剑波, 姜全, 陈晓玲. 桃花粉低温和超低温保存方法比较研究. 植物遗传资源学报, 2017, 18(4): 670-675Zhang J M, Yan W J, Li X, Huang B, Zhang H J, Zhao J B, Jiang Q, Chen X L. Study on low temperature storage and cryopreservation of pollen for peach germplasm resources. Journal of Plant Genetic Resources, 2017, 18(4): 670-675
    [37] Zhang D, Cervantes J, Huamán Z, Carey E, Ghislain M. Assessing genetic diversity of sweet potato (Ipomoea batatas (L.)Lam.) cultivars from tropical America using AFLP. Genetic Resources and Crop Evolution, 2000, 47(6): 659-665
    [38] Bakoumé C, Wickneswari R, Siju S, Rajanaidu N, Kushairi A, Billotte N. Genetic diversity of the world’s largest oil palm (Elaeis guineensis Jacq.) field genebank accessions using microsatellite markers. Genetic Resources and Crop Evolution, 2014, 62(3): 349-360
    [39] Rodriguez-bonilla L, Cuevas H E, Montero-rojas M, Bird-pico F, Luciano-rosario D, Siritunga D. Assessment of genetic diversity of sweet potato in Puerto Rico. PLoS ONE, 2014, 9(12): e116184
    [40] Ithnin M, Teh C K, Ratnam W. Genetic diversity of Elaeis oleifera (HBK) Cortes populations using cross species SSRs: Implication’s for germplasm utilization and conservation. BMC Genetics, 2017, 18:37
    [41] Olasupo F O, Adewale D B, Aikpokpodion P O, Muyiwa A A, Bhattacharjee R, Gutierrez O A, Motamayor J C, Schnell R J, Ebai S, Zhang D. Genetic identity and diversity of Nigerian cacao genebank collections verified by single nucleotide polymorphisms (SNPs): A guide to field genebank management and utilization. Tree Genetics and Genomes, 2018, 14: 32
    [42] Nandwani D. Genetic diversity in horticultural plants. Cham: Springer, 2019:87-125
    [43] 王力荣, 朱更瑞, 方伟超. 中国桃遗传资源. 北京: 中国农业出版社, 2012Wang L R, Zhu G R, Fang W C. Peach genetic resource in China. Beijing: China Agriculture Press, 2012
    [44] 任国慧, 吴伟民, 房经贵, 宋长年. 我国葡萄国家级种质资源圃的建设现状. 江西农业学报, 2012, 24(7): 10-13Ren G H, Wu W M, Fang J G, Song C N. Construction status of national grape germplasm resource nurseries in China. Acta Agriculturae Jiangxi, 2012,24(7):10-13
    [45] 王文辉, 王国平, 田路明, 李秀根, 吕晓兰, 张玉星, 张江红, 曹玉芬. 新中国果树科学研究70年—梨. 果树学报, 2019, 36(10): 1273-1282Wang W H, Wang G P, Tian L M, Li X G, Lv X L, Zhang Y X, Zhang J H, Cao Y F. Fruit scientific research in New China in the past 70 years: Pear. Journal of Fruit Science, 2019, 36(10): 1273-1282
    [46] 邓秀新, 束怀瑞, 郝玉金, 徐强, 韩明玉, 张绍铃, 段常青, 姜全, 易干军, 陈厚彬. 果树学科百年发展回顾. 农学学报, 2018(1):24-34.Deng X X, Shu H R, Hao Y J, Xu Q, Han M Y, Zhang S L, Duan C Q, Jiang Q, Yi G J, Chen H B. Review on the centennial development of pomology in China. Journal of Agriculture, 2018(1): 24-34
    [47] 邓秀新, 王力荣, 李绍华, 张绍铃, 张志宏, 丛佩华, 易干军, 陈学森, 陈厚彬, 钟彩虹. 果树育种40年回顾与展望. 果树学报, 2019, 36(4): 514-520Deng X X, Wang L R, Li S H, Zhang S L, Zhang Z H, Cong P H, Yi G J, Chen X S, Chen H B, Zhong C H. Retrospection and prospect of fruit breeding for last four decades in China. Journal of Fruit Science, 2019, 36(4): 514-520
    [48] 王力荣,吴金龙. 中国果树种质资源研究与新品种选育70年.园艺学报,2021, 48(4): 749-758Wang L R, Wu J L. Review for the research of fruit tree germplasm and breeding of new varieties in the past seven decades in China.Acta Horticulturae Sinica,2021,48(4): 749-758
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卢新雄,王力荣,辛霞,等.种质圃作物种质资源安全保存策略与实践[J].植物遗传资源学报,2023,24(1):32-43.

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  • 在线发布日期: 2023-01-12
  • 出版日期: 2023-01-12
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