苏州市农业科学院,江苏苏州 215106
研究方向为蔬菜种质创新及栽培技术研究,E-mail: guoqin1981@163.com
牟建梅,研究方向为蔬菜种质创新及栽培技术研究,E-mail: thmjm@163.com
作物生物学国家重点实验室开放课题(2021KF12)
Suzhou Academy of Agricultural Sciences, Jiangsu Suzhou 215106
Foundation project: Open Project of the State Key Laboratory of Crop Biology (2021KF12)
黄秋葵果实发育过程质地易木质化变硬,严重影响商品价值。为探究黄秋葵果实质地变硬的机制,选择易老化变硬的Z06和不易老化的苏优葵3号两个品种在其果实3个发育时期进行生化指标的测定及转录组测序。结果发现,品种间或不同发育时期果实质地的差异主要是由于木质素积累导致的木质化,纤维素和原果胶同时也起到积极的作用。转录组学分析表明,相同品种在不同果实发育时期的差异表达基因(DEGs,differentially expressed genes)主要在苯丙烷生物合成和次级代谢产物的生物合成途径显著富集;而相同时期两品种间的差异表达基因除了与苯丙烷生物合成途径有关,光合作用和光合作用天线蛋白途径也起重要作用。在果实成熟质地变硬阶段,苯丙氨酸酶(PAL,phenylalanineammonialyase)基因是影响木质素积累的关键基因;蔗糖合成酶3(SUS3,sucrose synthase)基因对纤维素的积累贡献最大,而β-葡萄糖苷酶(BGLU,β?-D-glucosidase)基因的下调表达也是促进纤维积累的重要因素;半乳糖醛酸转移酶6(GAUT6,galacturonosyltransferase)基因和SUS6基因对原果胶的积累贡献较大,但大部分果胶酯酶(PME,pectinesterase)基因和聚半乳糖醛酸酶(PG,polygalacturonase)基因对原果胶的积累均呈负贡献。木质素合成相关基因PAL6、PAL5、PAL1及肉桂酰辅酶A还原酶2(CCR2,cinnamoyl-CoA reductase)基因、细胞色素P450亚酶84A1(CYP84A1,cytochrome P450)基因、CYP73A12,以及光合途径相关基因放氧增强蛋白2(PSBP2,oxygen-evolving-complex)基因和叶绿素a/b结合蛋白2(CAB1R,chlorophyll a/b binding protein)基因是影响品种质地差异的重要基因。
The okra fruit is easily lignified, whereas the fruit lignification would decrease the commercial value. In order to explore the aging mechanism of okra fruit, two cultivars, Z06 (easy to age), and Suyoukui 3 (not easy to age), were used for physiological indicators quantification and transcriptome sequencing at three developmental stages. The differences in fruit texture between cultivars or at different developmental stages was mainly caused by the accumulation of lignin, while cellulose and protopectin were also found with positive effects. The differentially expressed genes (DEGs) of the same cultivar at different fruit development stages were significantly enriched in the phenylpropane biosynthesis and secondary metabolite biosynthesis pathways. While the DEGs between the cultivars were found with enrichments in the phenylpropane biosynthesis pathway, the photosynthesis and photosynthesis antenna protein pathways. During the hardening stage of okra fruit, the gene PAL was found as key regulator that affected lignin accumulation. The SUS3 gene contributed predominantly to the accumulation of cellulose, and the down-regulated expression of the BGLU gene was also an important factor in promoting the accumulation of cellulose. Two genes GAUT6 and SUS6 largely contributed to the accumulation of protopectin, but most of the PME and PG genes had a negative contribution to the accumulation of protopectin. Lignin synthesis-related genes PAL6, PAL5, PAL1, CCR2, CYP84A1, CYP73A12, and photosynthetic pathway-related genes PSBP2 and CAB1R were important genes that affected the texture differences of two cultivars.
张国芹,牟建梅,陈虎根.黄秋葵果实质地变化的生化和转录组联合分析[J].植物遗传资源学报,2023,24(1):282-295.
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