As a very valuable germplasm resource， the wheat materials showing male sterile is of significance in the study of wheat hybrid breeding and heterosis utilization. In this study， we identified a male sterility mutant 4167ms from a wheat high generation line 4167， followed by the phenotype classification， genetic analysis and molecular marker mapping. The anthers of the mutant were shriveled and not exposed. The pollens were irregular in shape and failed to be stained with 1% I₂-KI， indicating complete sterility of the pollens. Gained from the outcome of field trials over several years， the male sterility were stable without affects due to light and temperature conditions. The F₁ plants derived from crosses between 4167ms and a number of varieties were completely fertile. The segregation ratio （fertile vs. sterile plants） in F₂ populations fitted to 3∶1. The F₁ plants （KD342//4167ms/4167）， which were derived from reciprocal cross， were fertile， and the segregation ratio （fertile vs. sterile plants） in derived F₂ population of segregation was 3∶1. These results indicated that the sterility was controlled by a single recessive nuclear male sterile gene， temporarily designated ms1t. By taking use of a 4167ms/Chinese spring F₂ mapping population， together with bulked segregant analysis （BSA） and mapping with SSR molecular markers， the ms1t was delimited with five markers （Xwmc617-Xkd661-Xkd696-ms1t-Xkd495-Xkd393） on chromosome 4BS. The genetic distance of the closest flanking markers Xkd696 and Xkd495 to ms1t were 3.9 cM and 1.9 cM， respectively. The comparative analysis based on the physical map of Chinese Spring suggested that ms1t was allocated to the same genetic interval of the former classified ms1 locus， implying ms1t in mutant 4167ms as a novel recessive allele of the MS1gene.